EMAGE mouse gene expression spatial database

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EMAGE:18361

Ndufv2 NADH dehydrogenase (ubiquinone) flavoprotein 2 ( MGI:1920150)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_014298_01	euxassay_014298_02	euxassay_014298_03	euxassay_014298_04	euxassay_014298_05

euxassay_014298_06	euxassay_014298_07	euxassay_014298_08	euxassay_014298_09	euxassay_014298_10

euxassay_014298_11	euxassay_014298_12	euxassay_014298_13	euxassay_014298_14	euxassay_014298_15

euxassay_014298_16	euxassay_014298_17	euxassay_014298_18	euxassay_014298_19	euxassay_014298_20

euxassay_014298_21	euxassay_014298_22	euxassay_014298_23	euxassay_014298_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18361_wholemount_strong.wlz

18361_wholemount_moderate.wlz

18361_wholemount_weak.wlz

18361_wholemount_possible.wlz

18361_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18361_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

humerus

moderate

regional

moderate expression: see section 01 02 03 04 weak expression: see section 20 21 22 23 24

fibula

moderate

regional

moderate expression: see section 04

tibia

moderate

regional

moderate expression: see section 04 weak expression: see section 03 05 06

femur

moderate

regional

moderate expression: see section 05 06 07 08 weak expression: see section 09 20 21 22 24

meckel's cartilage

moderate

regional

moderate expression: see section 05 06 07 18 19 20 weak expression: see section 08 13 14 16 17

temporal bone petrous part

weak

regional

weak expression: see section 01 02

orbito-sphenoid

moderate

regional

moderate expression: see section 20 weak expression: see section 01 02 06 07 08 18 19

viscerocranium

moderate

regional

Expression in the turbinate bone.

scapula

moderate

regional

moderate expression: see section 01 02 03 04 20 21

pelvic girdle skeleton

moderate

regional

moderate expression: see section 12 19 weak expression: see section 11 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31479
Entity Detected:	Ndufv2, NADH dehydrogenase (ubiquinone) flavoprotein 2 ( MGI:1920150)
Sequence:	sense strand is shown >T31479 ATGTTCTCCTTGGCGCTGCGGGCCAGGGCGACCGGCCTCGCTGCTCAGTGGGGAAGACATGCAAGGAATT TGCATAAGACAGCAGTGCACAATGGTGCTGGAGGAGCCTTATTTGTGCATAGAGATACTCCTGAGAATAA CCCAGATACTCCATTTGATTTCACACCAGAAAACTATAAGAGGATAGAGGCAATAGTAAAAAACTACCCA GAAGGGCATCAAGCCGCTGCTGTGCTTCCAGTCCTGGATCTCGCCCAAAGGCAGAATGGATGGCTACCTA TCTCCGCTATGAACAAGGTGGCTGAAGTTTTACAAGTACCTCCAATGAGAGTATATGAAGTAGCAACTTT TTATACAATGTATAATCGAAAGCCAGTTGGGAAGTACCATATCCAGGTCTGCACTACTACACCTTGCATG CTGCGAGATTCTGACAGCATATTGGAGACCCTTCAGAGAAAGCTTGGAATAAAGGTTGGAGAGACTACAC CTGACAAACTTTTCACTCTTATAGAAGTGGAATGTTTAGGGGCCTGTGTAAATGCACCGATGGTTCAAAT AAATGACAACTACTATGAGGATCTGACACCCAAGGATATTGAAGAGATTATTGATGAACTCAAAGCTGGA AAAGTTCCCAAACCAGGGCCAAGGAGTGGCCGCTTCTGTTGTGAGCCAGCTGGAGGCCTTACTTCTTTGA CTGAACCACCCAAAGGACCTGGCTTTGGTGTGCAAGCAGGCCTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5003524), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 26983. Forward Primer - name:026983_F_IRAV63-66_A05_Ndufv2, sequence:ATGTTCTCCTTGGCGCTG; Reverse Primer - name:026983_R_SP6_IRAV63-66_A05_Ndufv2, sequence:AAAGGCCTGCTTGCACAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18357 same embryo
	EMAGE:18359 same embryo
	EMAGE:18358 same embryo
	EMAGE:18360 same embryo
	EurExpress:euxassay_014298 same experiment
	MGI:4826650 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source