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EMAGE:18362

Plekhb1 pleckstrin homology domain containing, family B (evectins) member 1 ( MGI:1351469)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18362 EMAGE:18362 EMAGE:18362 EMAGE:18362 EMAGE:18362
"Pseudo-wholemount" of euxassay_014317. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014317_01 euxassay_014317_02 euxassay_014317_03 euxassay_014317_04
EMAGE:18362 EMAGE:18362 EMAGE:18362 EMAGE:18362 EMAGE:18362
euxassay_014317_05 euxassay_014317_06 euxassay_014317_07 euxassay_014317_08 euxassay_014317_09
EMAGE:18362 EMAGE:18362 EMAGE:18362 EMAGE:18362 EMAGE:18362
euxassay_014317_10 euxassay_014317_11 euxassay_014317_12 euxassay_014317_13 euxassay_014317_14
EMAGE:18362 EMAGE:18362 EMAGE:18362 EMAGE:18362 EMAGE:18362
euxassay_014317_15 euxassay_014317_16 euxassay_014317_17 euxassay_014317_18 euxassay_014317_19
EMAGE:18362 EMAGE:18362 EMAGE:18362 EMAGE:18362 EMAGE:18362
euxassay_014317_20 euxassay_014317_21 euxassay_014317_22 euxassay_014317_23 euxassay_014317_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18362Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18362_wholemount_strong.wlz
18362_wholemount_moderate.wlz
18362_wholemount_weak.wlz
18362_wholemount_possible.wlz
18362_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18362_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
rib
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 21 22 23 24
radius
strong strong
regionalstrong expression: see section 24
ulna
strong strong
regionalstrong expression: see section 24
humerus
strong strong
regionalstrong expression: see section 01 02 03 04 20 21 22 23 24
hindlimb digit 2 phalanx
strong strong
regionalstrong expression: see section 22
hindlimb digit 3 metatarsal
strong strong
regionalstrong expression: see section 01
hindlimb digit 3 phalanx
strong strong
regionalstrong expression: see section 22
hindlimb digit 4 phalanx
strong strong
regionalstrong expression: see section 22
fibula
strong strong
regionalstrong expression: see section 01 02 23 24
tibia
strong strong
regionalstrong expression: see section 01 02 23 24
femur
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 18 19 20 21 22 23 24
otic capsule
strong strong
regionalstrong expression: see section 07 08 16 17
nasal septum
strong strong
regionalstrong expression: see section 12
viscerocranium
strong strong
regionalExpression in the turbinate bone.
hyoid
strong strong
regionalstrong expression: see section 11 12 13 14 15
meckel's cartilage
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
axial skeleton
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17
basioccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22
basisphenoid bone
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15
temporal bone petrous part
strong strong
regionalstrong expression: see section 01 02 03 04 19 20 21 22 23
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 17 18 19 20 21 22 23
clavicle
strong strong
regionalstrong expression: see section 05 06 07 16 17 18 19
scapula
strong strong
regionalstrong expression: see section 03 04 20 21 22
pelvic girdle skeleton
strong strong
regionalstrong expression: see section 08 09 10 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31489
Entity Detected:Plekhb1, pleckstrin homology domain containing, family B (evectins) member 1 ( MGI:1351469)
Sequence:sense strand is shown

>T31489
TCCGCAGCTACTATGGGCCACCTTATGCAGGTCCTGGTGTGACACACGTGATAGTTCGAGAGGACCCCTG
CTACAGCTCAGGCGCCCCTTTGGCCATGGGCATGCTTGCTGGGGCTGCCACGGGTGCTGCTCTTGGTTCA
CTTATGTGGTCGCCTTGTTGGTTCTGAGCCCTGGGGCTCTGACTTCTGGATGTGCATGTAGAACACAAGA
AGACCCTTTTCTTCTGGATCCTCTGCTAGCTACCATCTAAACTGTCCCATTTTGACCCCTTTCTCCCCAT
TAAGCCCTCTACGCTTGGCCCATTACTCCCACCATTTATCCTCTTTTCCGTCTCAAAGGAAGCTAACATC
TCAGGCACAGACATAGCCAAAAGTCCATACACCCATTGCCAGGCACTCCCATAAGTCACATGTGAATTTT
CTCTACACATAGCAAACATAACTTCATTTGGCTCTAAGTGTCTGAGGAATGGAGCTCACAAGAGAGCAAA
CAACAAAGCTACAGTTATCTGTCTGGTAAGGATAAGTCGGGGCAAGGAGCGAGGTCTGGGTGCCCAAAGA
ATCAAATAACAGTTGGCTTGGTGTTGTGGCAGGGGTTGGTTGTAGTGGTTCTAGCTCTATGTGAAGGAGC
TTGCTGGATAAAGGCACGATGGGGCTTTTTATGGAGTAAAGTTTCTTGATTTCAACTATAATGAATACTT
TGCCTGTCTAGTTAGTACTGGCAGGACATACCCTTAACCAAAACATGGAATTTGGCACCTTTTCCTCACT
TGTAGCTTCCCTATGATAGCTGAGCTAACCCTAAGGTTTCCTAGCTGTGGGAAGAGGTGAGTTCTTTGAG
ACCTCCGGCCTTAGGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5369461), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 28319. Forward Primer - name:028319_F_IRAV63-66_F22_Plekhb1, sequence:TCCGCAGCTACTATGGGC; Reverse Primer - name:028319_R_SP6_IRAV63-66_F22_Plekhb1, sequence:TTCCTAAGGCCGGAGGTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18366 same embryo
 EMAGE:18365 same embryo
 EMAGE:18364 same embryo
 EMAGE:18367 same embryo
 EMAGE:18363 same embryo
 EurExpress:euxassay_014317 same experiment
 MGI:4827269 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS