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EMAGE:18421

BC051142 cDNA sequence BC051142 ( MGI:3039565)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18421 EMAGE:18421 EMAGE:18421 EMAGE:18421 EMAGE:18421
"Pseudo-wholemount" of euxassay_014368. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014368_01 euxassay_014368_02 euxassay_014368_03 euxassay_014368_04
EMAGE:18421 EMAGE:18421 EMAGE:18421 EMAGE:18421 EMAGE:18421
euxassay_014368_05 euxassay_014368_06 euxassay_014368_07 euxassay_014368_08 euxassay_014368_09
EMAGE:18421 EMAGE:18421 EMAGE:18421 EMAGE:18421 EMAGE:18421
euxassay_014368_10 euxassay_014368_11 euxassay_014368_12 euxassay_014368_13 euxassay_014368_14
EMAGE:18421 EMAGE:18421 EMAGE:18421 EMAGE:18421 EMAGE:18421
euxassay_014368_15 euxassay_014368_16 euxassay_014368_17 euxassay_014368_18 euxassay_014368_19
EMAGE:18421 EMAGE:18421 EMAGE:18421 EMAGE:18421 EMAGE:18421
euxassay_014368_20 euxassay_014368_21 euxassay_014368_22 euxassay_014368_23 euxassay_014368_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18421Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18421_wholemount_strong.wlz
18421_wholemount_moderate.wlz
18421_wholemount_weak.wlz
18421_wholemount_possible.wlz
18421_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18421_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
facial vii ganglion
strong strong
regionalstrong expression: see section 05 06 21 22
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 08 19 20
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 17 18 19 20 21 22
vagus x ganglion
strong strong
regionalstrong expression: see section 08 19
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 07 08 18 19 20 21
trigeminal v nerve
strong strong
regionalstrong expression: see section 09 17
spinal cord
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 10 17 18
cervical ganglion
strong strong
regionalstrong expression: see section 09 18
thoracic ganglion
strong strong
regionalstrong expression: see section 13 14
dorsal root ganglion
strong strong
regionalstrong expression: see section 08 09 10 11 15 16 17 18 19 20
neural retina
strong strong
regionalstrong expression: see section 01 02 03 23 24
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 14 15 16
vomeronasal organ
strong strong
regionalstrong expression: see section 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31856
Entity Detected:BC051142, cDNA sequence BC051142 ( MGI:3039565)
Sequence:sense strand is shown

>T31856
GTGCAGTTCTCGGCACCTATTCCCGGAGCTACAGGACCTATCAAACTGACTCAAAAAACCTTCCTGCAAA
CTCCTGGACCTATTGTACAATGTATGGATTCCAGTGAAAGCTGCACCCCTGAGGGATCCAACGCTGTAAT
ACCTGTGGACATATGTTGTCAACCAGCACTGAAGCCTAGCTGTTGTCAACAGCTAGCAATCAAACCTAGC
TGTTGTCAGCCTTGTCAGCCTTGTCAGCCTTGTCAGCCTTATCAACCTTGTCAGCCTTCTCCGCCAGACA
CCTTGCCCTTATTAATTACACAGAGAACTTCAATGAAAAAGCCATCAGATTGCAAAGGCCAGATGGTAAG
ACATGGAGGGAGTGTAACCTTCCAGGGTATCGGAGATGACCAAATAAAGTGCTGTAAGGAGTGGAGAAGA
TGCCAGTGTGAATACTGCTGTCCAAAGAGATTCGAGCCCCACAGACGAGGAACCATATGGTGTGAACAAC
CAGCTCCTTCCTCTTCTCATCAGCATCAGCATCAGCAGCAGCAGCAGCAGCAGCCACCACCGCCGCCGCC
TCCACCACGAAGTCAAGATGCTCAAAAGACTGGAGTCCCTGATAAGGAGGAACCAAGACGGGATAGTAAT
AAAGCAGAATGAAGATGGCTTTCTGATAAAGAAGGCAGAGGAAGGCAAAAAGAAAAAGAAAACATCCAGG
AGGGCATCAGCACACCCAGAAACAGATTCTGATTCCAGCGCTCCACAGACCGGAGAGGCTCCTTTCGAAA
GTAAACCACCCGAAGAACAACCAGAGGGAGAAGAGAAGAAACGCAAGAAAGAAAAGAAGAAAAAGAAGAA
AAAAGACAAAGACGGCTGAGAAAGGAGGAGGAGGACAGGGCAAAACATGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6740688), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61020. Forward Primer - name:061020_F_IRAW2_d04_BC051142, sequence:GTGCAGTTCTCGGCACCT; Reverse Primer - name:061020_R_SP6_IRAW2_d04_BC051142, sequence:GCCATGTTTTGCCCTGTC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18423 same embryo
 EMAGE:18420 same embryo
 EMAGE:18419 same embryo
 EMAGE:18422 same embryo
 EMAGE:18418 same embryo
 EurExpress:euxassay_014368 same experiment
 MGI:4823429 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS