EMAGE mouse gene expression spatial database

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EMAGE:18481

Rab11fip2 RAB11 family interacting protein 2 (class I) ( MGI:1922248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014448. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014448_01	euxassay_014448_02	euxassay_014448_03	euxassay_014448_04

euxassay_014448_05	euxassay_014448_06	euxassay_014448_07	euxassay_014448_08	euxassay_014448_09

euxassay_014448_10	euxassay_014448_11	euxassay_014448_12	euxassay_014448_13	euxassay_014448_14

euxassay_014448_15	euxassay_014448_16	euxassay_014448_17	euxassay_014448_18	euxassay_014448_19

euxassay_014448_20	euxassay_014448_21	euxassay_014448_22	euxassay_014448_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18481_wholemount_strong.wlz

18481_wholemount_moderate.wlz

18481_wholemount_weak.wlz

18481_wholemount_possible.wlz

18481_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18481_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

moderate

regional

moderate expression: see section 04 15 16 17 weak expression: see section 03 05 06 07 08 09

male reproductive system

weak

regional

weak expression: see section 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37965
Entity Detected:	Rab11fip2, RAB11 family interacting protein 2 (class I) ( MGI:1922248)
Sequence:	sense strand is shown >T37965 GGGTGTTTTCTGATACGTCCTCTGCCATCGTTCCGAGCACTCACATGCCTGATGCCAATCCTGAGTTTTC AAGTGGTGAAATGCAGATGAAATCCAAACCAAAAAAGCCTTTTCTTTTGGGTCCTCAGCGGCTCTCTTCT GCCCATTCGATGTCTGATTTAACTGGGTCCCACTTATCTTCTGAGAAGCTGAAGTCCAGCACTGTGGGTC CAACACATCTTCTCAGTCGCCAGATAGATTCCTTTGGAGTTGTTCCAGAAAGTGGAAGTCTCAAGTCTCC ACACAGACGAACACTAAGCTTTGATACTTCTAAATTGAACCAACCTGGCAGCATTGTGGATGAAGGTGAA CACTCTTTTGGAAGACAGAGTGACCCATTTACAAATGTGACTGCTTCATTACCCCAAAAATTTGCAACAC TGCCAAGGAAGAAGAATCCATTTGAAGAAAGCAGTGAGCCATGGGACAGCAGCATGAATTTATTCTCAAA ACCAATTGAAGTCAGGAAAGAAAGTAAGAGAGAGAAAAGGGAGAAGGTGAGCCTCTTTGAAAGAGTGACT GGCAAGAGAGATAGCAGGAGACCTGACAAGCTGAACAATGGTGGATCTGATAGCCCATGTGACTTGAAAT CACCTAGTGCTTTTAGTGAAAACCGGCAGGACTATTTTGAATATGAGTCAACTAACCCGTTTACAGCAAA ATTCAGGGCTTCAACTATAATGCCATCTTCAAGTTTTCATGTGAATCCAACAAGCAGTGAAGACCTCAGG AAAATCCCGGACAACAATCCTTTCGATGCCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 86251. Forward Primer - name:086251_F_cDNA_Rab11fip2, sequence:GGGTGTTTTCTGATACGTCCTC; Reverse Primer - name:086251_N_SP6_cDNA_Rab11fip2, sequence:GTGGCATCGAAAGGATTGTTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18486 same embryo
	EMAGE:18484 same embryo
	EMAGE:18483 same embryo
	EMAGE:18482 same embryo
	EMAGE:18485 same embryo
	EurExpress:euxassay_014448 same experiment
	MGI:4827563 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source