EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18483

Glcci1 glucocorticoid induced transcript 1 ( MGI:2179717)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014439. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014439_01	euxassay_014439_02	euxassay_014439_03	euxassay_014439_04

euxassay_014439_05	euxassay_014439_06	euxassay_014439_07	euxassay_014439_08	euxassay_014439_09

euxassay_014439_10	euxassay_014439_11	euxassay_014439_12	euxassay_014439_13	euxassay_014439_14

euxassay_014439_15	euxassay_014439_16	euxassay_014439_17	euxassay_014439_18	euxassay_014439_19

euxassay_014439_20	euxassay_014439_21	euxassay_014439_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18483_wholemount_strong.wlz

18483_wholemount_moderate.wlz

18483_wholemount_weak.wlz

18483_wholemount_possible.wlz

18483_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18483_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 12 13 14 15 16 moderate expression: see section 02 17

midbrain ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 weak expression: see section 03 04 05 10 11

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 14 weak expression: see section 05

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 08 09 10 11 12 14 15

nasal septum

weak

regional

weak expression: see section 13

vomeronasal organ

weak

regional

weak expression: see section 11 13

axial skeleton

moderate

regional

moderate expression: see section 08 weak expression: see section 09 10 11 12 13

axial skeleton tail region

weak

regional

weak expression: see section 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31884
Entity Detected:	Glcci1, glucocorticoid induced transcript 1 ( MGI:2179717)
Sequence:	sense strand is shown >T31884 ACGGGACCCTCATGTTCACTACCCTTCATGCATGAGAGATAAAGCCACTCAGACACCTAGTTGCTGGGCA GAAGAGGGAGCAGAAAAACGATCACATCAGCGCTCTGCATCATGGGGAAGTGCTGATCAACTGAAAGAGC AGATTGCCAAACTTAGGCAACAGCTACAACGCAGCAAGCAAAGTAGTCGGCATAGTAAAGAGAAGGACAG ACAGTCACCTCTCCATGGCAACCACATAACAATCAGTCATACTCAGGCTATTGGATCAAGATCAGTCCCT ATGCCTCTGTCAAACATATCAGTGCCAAAATCATCTGTTTCCCGTGTACCCTGCAATGTAGAAGGAATAA GTCCTGAATTGGAAAAGGTATTCATCAAAGAAAACAATGGAAAGGAAGAAGTGTCCAAGCCATTGGATAT ACCAGATGGTCGAAGAGCTCCGCTCCCTGCTCACTACAGGAGCAGTAGTACTCGAAGCATAGATACCCAG ACACCTTCTGTCCAAGAGCGCAGCAGTAGCTGCAGCAGCCACTCCCCTTGTGTGTCCCCATTTTGTCCTC CGGAATCCCAGGATGGAAGTCCTTGTTCAACAGAAGATTTGCTCTATGATCGTGATAAAGACAGTGGGAG TAGCTCACCGTTACCCAAGTATGCTTCATCTCCCAAACCCAACAACAGCTACATGTTCAAACGGGAGCCC CCAGAGGGATGTGAGCGGGTGAAGGTCTTTGAGGAAATGGCGTCTCGTCAGCCTATCTCGGCCCCTCTCT TTTCATGTCCTGACAAAAACAAGGTTAATTTCATCCCAACCGGATCAGCTTTCTGTCCTGTAAAACTTCT AGGCCCTCTCTTACCTGCCTCTGACCTGATGCTCAAGAACTCCCCTAATTCTGGCCAGAGCTCGGCTCTG GCAACAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6771009), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 61139. Forward Primer - name:061139_F_IRAW3_f03_Glcci1, sequence:ACGGGACCCTCATGTTCA; Reverse Primer - name:061139_R_SP6_IRAW3_f03_Glcci1, sequence:AGTGTTGCCAGAGCCGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18486 same embryo
	EMAGE:18484 same embryo
	EMAGE:18482 same embryo
	EMAGE:18485 same embryo
	EMAGE:18481 same embryo
	EurExpress:euxassay_014439 same experiment
	MGI:4825069 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source