EMAGE mouse gene expression spatial database

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EMAGE:18513

S100a7a S100 calcium binding protein A7A ( MGI:2687194)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014464. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014464_01	euxassay_014464_02	euxassay_014464_03	euxassay_014464_04

euxassay_014464_05	euxassay_014464_06	euxassay_014464_07	euxassay_014464_08	euxassay_014464_09

euxassay_014464_10	euxassay_014464_11	euxassay_014464_12	euxassay_014464_13	euxassay_014464_14

euxassay_014464_15	euxassay_014464_16	euxassay_014464_17	euxassay_014464_18	euxassay_014464_19

euxassay_014464_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18513_wholemount_strong.wlz

18513_wholemount_moderate.wlz

18513_wholemount_weak.wlz

18513_wholemount_possible.wlz

18513_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18513_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

single cell

weak expression: see section 05 06

pons mantle layer

weak

single cell

weak expression: see section 05 14

facial vii ganglion

weak

single cell

weak expression: see section 14

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 13 weak expression: see section 14

trigeminal v ganglion

moderate

single cell

moderate expression: see section 15 16 weak expression: see section 05 06 14

vestibulocochlear viii ganglion

moderate

single cell

moderate expression: see section 13 weak expression: see section 05

ventral grey horn

moderate

single cell

moderate expression: see section 05 06 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37985
Entity Detected:	S100a7a, S100 calcium binding protein A7A ( MGI:2687194)
Sequence:	sense strand is shown >T37985 AGGACTCCCTCTTCCAAATCATACACTGCTTCCATCACTATGCTGCCCGGGAAGGGGACAAGGAGACCTT GTCCCTGGAGGAGTTGAAAGCTCTGCTCTTGGATAGTGTGCCTCGCTTCATGGACACCTTGGGCCGCAGG CAGCCATACTACATCACAGAGCTGTTCCGGGCAGCTGACAAAAACAAGGACAACCAGATCTGCTTTGATG AGTTCCTGTACATCTTGGGCAAGCTGGTGAAGGACTACCATCTCCAGTTCCACCGGCAGTTGTGTGCACA CTACTGTACTGAGCACAGCCTCTACTAGAGGGAGGACAGGCAGTCTCTCATCACCAGAACCTCTCCCGAG ACATGGCCTGATCATGAACAGTGTACAGAACTCAGCAATGTATTTCTTCTTAGGTGTGTGCACATGCATA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96429. Forward Primer - name:096429_F_cDNA_S100a15, sequence:AGGACTCCCTCTTCCAAATCAT; Reverse Primer - name:096429_N_SP6_cDNA_S100a15, sequence:TATGCATGTGCACACACCTAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18514 same embryo
	EMAGE:18512 same embryo
	EMAGE:18511 same embryo
	EurExpress:euxassay_014464 same experiment
	MGI:4827853 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source