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EMAGE:18529

Slc22a3 solute carrier family 22 (organic cation transporter), member 3 ( MGI:1333817)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18529 EMAGE:18529 EMAGE:18529 EMAGE:18529 EMAGE:18529
"Pseudo-wholemount" of euxassay_014478. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014478_01 euxassay_014478_02 euxassay_014478_03 euxassay_014478_04
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euxassay_014478_05 euxassay_014478_06 euxassay_014478_07 euxassay_014478_08 euxassay_014478_09
EMAGE:18529 EMAGE:18529 EMAGE:18529 EMAGE:18529 EMAGE:18529
euxassay_014478_10 euxassay_014478_11 euxassay_014478_12 euxassay_014478_13 euxassay_014478_14
EMAGE:18529 EMAGE:18529 EMAGE:18529 EMAGE:18529 EMAGE:18529
euxassay_014478_15 euxassay_014478_16 euxassay_014478_17 euxassay_014478_18 euxassay_014478_19
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euxassay_014478_20 euxassay_014478_21 euxassay_014478_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18529Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18529_wholemount_strong.wlz
18529_wholemount_moderate.wlz
18529_wholemount_weak.wlz
18529_wholemount_possible.wlz
18529_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18529_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38003
Entity Detected:Slc22a3, solute carrier family 22 (organic cation transporter), member 3 ( MGI:1333817)
Sequence:sense strand is shown

>T38003
GACGTAGAAAAGCTTGGCAGTTCACAGTTGCATCAGTGTGGCAGAAAAAAGAAAACCCAGGTTTCTACTT
CTGATGTCTGAGGCCCCTGTGTTGCCTGGAGGACCAGAGAAGACTGGAATATGCCTGTGCAGTGTGCTTC
GCCTCCTCTAGGTGGCGCCCTTCCAAGGAACCGTAAAGATTTCGGAGTATTTTATAAGGTATAAGATGAA
GATTTATCATGCTGCCCAAGCTTCGGGGAACACATAATCTTGGACCTGTTTCATGAAGACCCAGTAGGTA
CCCGGATTCTGGCTAGGTTCTCATTCAGCAAGTCTCTCACCCATTAGGTAAGGAGAGATGAAAAGGCCCC
AACCAGTTCTCCAGTTCTCAGGACAAGACAGTACCATCCAGGAAGCAGGATGAACAAACCAAGGCCTGGC
ACGTGTCCAGAAGTGCGCTCAGCTGATCTGATCAACACACCGCAGTGAAGAGTGTTCTGAAGAGTCTGAG
AACTGCTATTTCTTCCCTTGAGCTCCGAATACAAGCCTTCGCAGGGCCGACATGTCCGGCGCTGCTCCCC
TCCTGTCTCCTGCAGGTGCTGAGGTCCCAGGTCCCAGGTTAGGCACAGCTCCTTAAATGCAACACAACAG
AGGACCTGGCTGTGGTATTGTGAAGTCATGCTGCAGTCCCCAGGCCCCAGATATTTCGCCACCTCCTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99464. Forward Primer - name:099464_F_cDNA_Slc22a3, sequence:GACGTAGAAAAGCTTGGCAGTT; Reverse Primer - name:099464_N_SP6_cDNA_Slc22a3, sequence:GAAGGAGGTGGCGAAATATCTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18528 same embryo
 EMAGE:18530 same embryo
 EMAGE:18533 same embryo
 EMAGE:18532 same embryo
 EMAGE:18531 same embryo
 EurExpress:euxassay_014478 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS