EMAGE mouse gene expression spatial database

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EMAGE:18532

Slc25a2 solute carrier family 25 (mitochondrial carrier, ornithine transporter) member 2 ( MGI:2137907)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014479. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014479_01	euxassay_014479_02	euxassay_014479_03	euxassay_014479_04

euxassay_014479_05	euxassay_014479_06	euxassay_014479_07	euxassay_014479_08	euxassay_014479_09

euxassay_014479_10	euxassay_014479_11	euxassay_014479_12	euxassay_014479_13	euxassay_014479_14

euxassay_014479_15	euxassay_014479_16	euxassay_014479_17	euxassay_014479_18	euxassay_014479_19

euxassay_014479_20	euxassay_014479_21	euxassay_014479_22	euxassay_014479_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18532_wholemount_strong.wlz

18532_wholemount_moderate.wlz

18532_wholemount_weak.wlz

18532_wholemount_possible.wlz

18532_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18532_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 08

pons mantle layer

strong

single cell

strong expression: see section 08 09 10 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38005
Entity Detected:	Slc25a2, solute carrier family 25 (mitochondrial carrier, ornithine transporter) member 2 ( MGI:2137907)
Sequence:	sense strand is shown >T38005 AAAAGAGACTTGGCTTCTGACGACACCCTCGGGAGGGTGGAGGAACATGAAGTCCACCCTGCCATCCAAG CCGCCATAGACCTCACTGCGGGCGCAGCAGGGGGCGGAGCTTGTGTGCTGACTGGGCAACCCTTCGACAC CATAAAAGTAAAGATGCAGACATTTCCTCAGCTGTACAAAGGCCTTGCCGACTGCTTCCTGAAAACATAC AACCAAGTGGGCATCCGTGGCCTTTACAGGGGAACCAGTCCTGCACTGCTAGCCTATGTCACCCAGGGTT CTGTCCTGTTCATGTGCTTTGGCTTTTGCCAACAGTTTGTCAGGAAAGTGGCTAGAGTGGAGCAGAATGC AGAGCTGAACGACTTGGAGACTGCTACTGCTGGGTCGCTGGCTTCTGCATTTGCTGCGCTGGCTCTCTGC CCCACTGAGCTTGTGAAGTGTCGGCTGCAGACCATGTATGAGATGAAGATGTCAGGGAAGATAGCACAAA GCTATAACACAATTTGGTCTATGGTTAAGAGTATCTTCATGAAGGATGGTCCCTTAGGCTTCTATCGTGG ACTCTCGACCACTCTTGCTCAGGAAATACCTGGCTATTTCTTCTACTTTGGGGGCTATGAAATCAGTCGA TCATTTTTTGCATCAGGGGGATCAAAGGATGAACTAGGCCCTGTCCCTTTGATGTTAAGTGGAGGCTTTG CTGGGATCTGTCTCTGGCTTATCATATTCCCAGTGGACTGCATTAAATCCAGAATCCAGGTTCTTTCTAT GTTTGGGAAGCCTGCAGGATTAATCGAAACCTTTATAAGTGTTGTGAGAAATGAAGGGATATCAGCCTTG TATTCTGGATTGAAAGCCACTCTGATTCGAGCCATCCCTTCCAATGCTGCTCTCTTTTTGGTTTATGAGT ACAGCAGAAAGATGATGATGAACATGGTGGAAGAATACTGAAGTGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85751. Forward Primer - name:085751_F_cDNA_Slc25a2, sequence:AAAAGAGACTTGGCTTCTGACG; Reverse Primer - name:085751_N_SP6_cDNA_Slc25a2, sequence:GACACTTCAGTATTCTTCCACCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18528 same embryo
	EMAGE:18530 same embryo
	EMAGE:18533 same embryo
	EMAGE:18529 same embryo
	EMAGE:18531 same embryo
	EurExpress:euxassay_014479 same experiment
	MGI:4828150 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source