EMAGE mouse gene expression spatial database

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EMAGE:18535

Slc26a4 solute carrier family 26, member 4 ( MGI:1346029)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014488. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014488_01	euxassay_014488_02	euxassay_014488_03	euxassay_014488_04

euxassay_014488_05	euxassay_014488_06	euxassay_014488_07	euxassay_014488_08	euxassay_014488_09

euxassay_014488_10	euxassay_014488_11	euxassay_014488_12	euxassay_014488_13	euxassay_014488_14

euxassay_014488_15	euxassay_014488_16	euxassay_014488_17	euxassay_014488_18	euxassay_014488_19

euxassay_014488_20	euxassay_014488_21	euxassay_014488_22	euxassay_014488_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18535_wholemount_strong.wlz

18535_wholemount_moderate.wlz

18535_wholemount_weak.wlz

18535_wholemount_possible.wlz

18535_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18535_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

weak

regional

weak expression: see section 09 10 11 12 13 14

trigeminal v ganglion

moderate

single cell

moderate expression: see section 17 18 20 weak expression: see section 19

spinal cord

weak

regional

weak expression: see section 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38007
Entity Detected:	Slc26a4, solute carrier family 26, member 4 ( MGI:1346029)
Sequence:	sense strand is shown >T38007 GTTTATGCAGGTGTGTGACGTTCCTCGTCTGTGGAAGCAGAATAAGACTGATGCTGTTATCTGGGTGTTT ACATGCATAATGTCCATCATTCTGGGGCTGGACCTCGGCTTGCTAGCTGGCCTTTTATTTGCACTACTGA CTGTGGTCCTGAGAGTTCAGTTCCCTTCATGGAATGGCCTTGGAAGTGTCCCCAGCACAGACATCTACAA AAGCATCACACATTATAAAAACCTTGAAGAGCCTGAAGGGGTGAAGATCCTGAGATTTTCCAGTCCCATT TTTTACGGCAATGTCGATGGTTTTAAAAAATGTATCAATTCAACGGTTGGATTTGATGCCATTAGAGTAT ATAATAAGAGGCTGAAAGCACTGAGAAGAATACAGAAACTCATCAAAAAAGGACAACTCAGGGCAACCAA GAACGGGATCATAAGTGATATTGGCTCATCAAATAATGCCTTCGAGCCTGATGAAGATGTGGAAGAGCCA GAGGAACTTAATATCCCAACCAAAGAAATTGAGATTCAAGTGGACTGGAACTCCGAACTCCCGGTGAAAG TGAATGTCCCAAAAGTGCCAATCCACAGCCTGGTGCTGGATTGCGGAGCTGTATCCTTCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 99599. Forward Primer - name:099599_F_cDNA_Slc26a4, sequence:GTTTATGCAGGTGTGTGACGTT; Reverse Primer - name:099599_N_SP6_cDNA_Slc26a4, sequence:GGAAGGATACAGCTCCGCAAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18536 same embryo
	EMAGE:18534 same embryo
	EMAGE:18538 same embryo
	EMAGE:18537 same embryo
	EurExpress:euxassay_014488 same experiment
	MGI:4828166 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source