EMAGE mouse gene expression spatial database

EMAGE:18566

Pcdha11 protocadherin alpha 11 ( MGI:1298372)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014511. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014511_01	euxassay_014511_02	euxassay_014511_03	euxassay_014511_04

euxassay_014511_05	euxassay_014511_06	euxassay_014511_07	euxassay_014511_08	euxassay_014511_09

euxassay_014511_10	euxassay_014511_11	euxassay_014511_12	euxassay_014511_13	euxassay_014511_14

euxassay_014511_15	euxassay_014511_16	euxassay_014511_17	euxassay_014511_18	euxassay_014511_19

euxassay_014511_20	euxassay_014511_21	euxassay_014511_22	euxassay_014511_23	euxassay_014511_24

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38560
Entity Detected:	Pcdha11, protocadherin alpha 11 ( MGI:1298372)
Sequence:	sense strand is shown >T38560 CTCTTTGATGTCCATTAAGCCCACTGGGAGGCATTTATTTACCCTAGATGAAAAAAATGGAGAATTGAGG GTCAACGGGACTTTAGATTATGAAGAGAACAAATTGTATGAAATAGAAGTGCTGGCCACGGATAAAGGAA CACCTCCAATGGTAGGTCACTGTGTAGTCTTGGTAGAAATCTTAGACACCAATGACAACTCCCCTGAAGT GACATTAACTTCGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 149921. Forward Primer - name:149921_F_cDNA_Pcdha11, sequence:CTCTTTGATGTCCATTAAGCCC; Reverse Primer - name:149921_N_SP6_cDNA_Pcdha11, sequence:GCGAAGTTAATGTCACTTCAGGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18567 same embryo
	EMAGE:18569 same embryo
	EMAGE:18570 same embryo
	EMAGE:18568 same embryo
	EMAGE:18571 same embryo
	EurExpress:euxassay_014511 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source