EMAGE mouse gene expression spatial database

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EMAGE:18624

Otop3 otopetrin 3 ( MGI:1916852)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016493. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016493_01	euxassay_016493_02	euxassay_016493_03	euxassay_016493_04

euxassay_016493_05	euxassay_016493_06	euxassay_016493_07	euxassay_016493_08	euxassay_016493_09

euxassay_016493_10	euxassay_016493_11	euxassay_016493_12	euxassay_016493_13	euxassay_016493_14

euxassay_016493_15	euxassay_016493_16	euxassay_016493_17	euxassay_016493_18	euxassay_016493_19

euxassay_016493_20	euxassay_016493_21	euxassay_016493_22	euxassay_016493_23	euxassay_016493_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18624_wholemount_strong.wlz

18624_wholemount_moderate.wlz

18624_wholemount_weak.wlz

18624_wholemount_possible.wlz

18624_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18624_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

weak

regional

weak expression: see section 08 12 13 14 15 16 17 18

telencephalon

moderate

regional

moderate expression: see section 01 02 03 04 05 06 19 20 21 22 23 24 weak expression: see section 07 08 12 13 14 15 16 17 18

hindbrain

moderate

regional

moderate expression: see section 19 20 weak expression: see section 03 04 05 06 07 08 12 13 14 15 16 17 18

midbrain

weak

regional

weak expression: see section 07 08 12 13 14 15 16 17 18

facial vii ganglion

weak

regional

weak expression: see section 04 19 20

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 08 17 18

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 17 18 19 20 21 22

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07

spinal cord

weak

regional

weak expression: see section 13 14

dorsal root ganglion

weak

spotted

weak expression: see section 07 08 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38715
Entity Detected:	Otop3, otopetrin 3 ( MGI:1916852)
Sequence:	sense strand is shown >T38715 TGCTCTGGAGACACTGTAAGGACTGTGTTCAGGTGCAGACCAACTTCACCAGGTGTGGACTCATGCTGAC TCTGGCCACAAACTTGCTGATGTGGGTTTTGGCTGTGACCAATGACTCCATGCACCGTGAGATTGAGGCT GAGCTCGACGCCCTCATGGAGAAGTTCTCAGGTAACGGGACCAACACTTGCATGTGCCTCAACACCACAG TGTGCGAGGTCTTCCGGAAGGGCTATCTGATGCTCTACCCCTTCAGCACTGAGTACTGCCTCATCTGCTG TGCTGTGCTCTTCGTCATGTGGAAGAACGTGAGCCGCAGCCTGGCAGCCCACACAGGTGCCCATCCCAAC AGATCACCCTTCCGCCTGCACGGGACCATCTTTGGGCCACTGCTGGGCCTTTTGGCACTGGTGGCTGGCG TATGTGTCTTTGTGCTCTTCCAAATCGAAGCGAGTGGCCCTGACATAGCCCGCCAGTACTTCACCCTCTA CTATGCGTTCTATGTGGCTGTGCTGCCCACCATGAGCCTGGCGTGCCTGGCTGGTACAGCCATCCATGGG CTGGAGGAGCGCGAATTGGACACGCTCAAAAACCCCACACGCAGCCTGGATGTGGTGCTGTTGATGGGCG CTGCACTGGGCCAGATGGGCATCGCCTACTTCTCCATCGTGGCTATCGTGGCCACCCAGCCGCACGAGCT ACTCAACCAGCTTATCCTGGCCTACTCACTGCTGCTAATCCTGCAGCACATCACTCAGAATCTCTTCATC ATCGAGGGCCTGCACCGCCGCCCCCTCTGGGAGCCTGCGGTCTCAGGTGTGATGGAGAAGCAGGATGTTG AGCTACCCCGCAGAGGCTCCCTGCGGGAGCTGGGTCAGGACCTACGGAGAGCCTCAAGGGCCTACATCCA CTCCTTCAGCCACCTCAACTGGAAAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 103782. Forward Primer - name:103782_F_cDNA_Otop3, sequence:TGCTCTGGAGACACTGTAAGGA; Reverse Primer - name:103782_N_SP6_cDNA_Otop3, sequence:GTTTCCAGTTGAGGTGGCTGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18623 same embryo
	EMAGE:18625 same embryo
	EMAGE:18626 same embryo
	EurExpress:euxassay_016493 same experiment
	MGI:4827004 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source