EMAGE mouse gene expression spatial database

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EMAGE:18636

Xpnpep2 X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound ( MGI:2180001)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016523. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016523_01	euxassay_016523_02	euxassay_016523_03	euxassay_016523_04

euxassay_016523_05	euxassay_016523_06	euxassay_016523_07	euxassay_016523_08	euxassay_016523_09

euxassay_016523_10	euxassay_016523_11	euxassay_016523_12	euxassay_016523_13	euxassay_016523_14

euxassay_016523_15	euxassay_016523_16	euxassay_016523_17	euxassay_016523_18	euxassay_016523_19

euxassay_016523_20	euxassay_016523_21	euxassay_016523_22	euxassay_016523_23	euxassay_016523_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18636_wholemount_strong.wlz

18636_wholemount_moderate.wlz

18636_wholemount_weak.wlz

18636_wholemount_possible.wlz

18636_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18636_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

weak

regional

weak expression: see section 14 15

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 09 weak expression: see section 04 05 06 07 08 11 12 17 18 20 21

olfactory cortex mantle layer

weak

regional

weak expression: see section 17

rest of cerebellum ventricular layer

weak

regional

weak expression: see section 08 12 17 18

midbrain mantle layer

weak

regional

weak expression: see section 06 07 13 14 15

midbrain ventricular layer

weak

regional

weak expression: see section 12

neural retina

moderate

regional

moderate expression: see section 23 weak expression: see section 22 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38736
Entity Detected:	Xpnpep2, X-prolyl aminopeptidase (aminopeptidase P) 2, membrane-bound ( MGI:2180001)
Sequence:	sense strand is shown >T38736 AGTGACATCCCCTATAACCCCTTCTTCTACTCCTATGCACTGCTCACGAACTCCTCCATCAGGTTGTTTG TCAACAAGAGTCGCTTTAGCCTTGAGACCTTGCAGTACCTGAATACAAACTGCACATTACCCATGTGTGT GCAACTTGAGGACTACAGTCAAGTTCGTGACAGTGTGAAGGCCTATGCCTCAGGCGATGTGAAAATCTTA ATTGGGGTCAGCTATACCACCTATGGGGTCTATGAAGTGATACCCAAGGAGAAACTCGTGACAGACACCT ACTCCCCAGTGATGTTAATCAAGGCTGTGAAGAACAGCAAGGAGCAGGCCCTTCTGAAGAGCAGCCACGT GCGGGACGCTGTGGCTGTGATCCAGTACTTGGTCTGGTTGGAGAAGAACGTGCCCAAAGGCACGGTGGAT GAGTTTTCTGGGGCAGAATACATTGATGAGTTACGACGGAATGAAAACTTCTCCTCTGGACCCAGTTTTG AAACCATCTCTGCTAGTGGCCTGAATGCCGCCCTGGCCCATTACAGCCCAACAAAAGAGCTGCACCGTAA GCTGTCCTCAGATGAGATGTACCTGGTGGATTCTGGGGGGCAATACTGGGATGGGACCACAGATATTACC AGAACAGTACATTGGGGCACTCCTACTGCCTTCCAAAAGGAGGCCTATACTCGAGTGCTAATGGGAAACA TCGATCTGTCCAGACTTGTCTTTCCTGCTGCTACATCAGGGAGAGTGATAGAAGCCTTTGCCCGAAGAGC CTTATGGGAAGTTGGGCTCAATTATGGTCATGGGACAGGCCATGGCATTGGCAACTTCCTCTGTGTGCAT GAGTGGCCAGTGGGATTCCAGTATAACAACATTGCCATGGCCAAGGGCATGTTCACTTCCATTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 104257. Forward Primer - name:104257_F_cDNA_Xpnpep2, sequence:AGTGACATCCCCTATAACCCCT; Reverse Primer - name:104257_N_SP6_cDNA_Xpnpep2, sequence:CAATGGAAGTGAACATGCCCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18637 same embryo
	EMAGE:18638 same embryo
	EurExpress:euxassay_016523 same experiment
	MGI:4829244 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source