EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18650

Psmd1 proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 ( MGI:1917497)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016545. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016545_01	euxassay_016545_02	euxassay_016545_03	euxassay_016545_04

euxassay_016545_05	euxassay_016545_06	euxassay_016545_07	euxassay_016545_08	euxassay_016545_09

euxassay_016545_10	euxassay_016545_11	euxassay_016545_12	euxassay_016545_13	euxassay_016545_14

euxassay_016545_15	euxassay_016545_16	euxassay_016545_17	euxassay_016545_18	euxassay_016545_19

euxassay_016545_20	euxassay_016545_21	euxassay_016545_22	euxassay_016545_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18650_wholemount_strong.wlz

18650_wholemount_moderate.wlz

18650_wholemount_weak.wlz

18650_wholemount_possible.wlz

18650_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18650_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 08 11 12

hypothalamus mantle layer

moderate

regional

moderate expression: see section 09 10 13 14

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 13 weak expression: see section 11

telencephalon mantle layer

strong

spotted

strong expression: see section 05 moderate expression: see section 04

ventral grey horn

weak

regional

weak expression: see section 07 08 10 11

dorsal root ganglion

weak

regional

weak expression: see section 05 06 07 12 13 14

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 11 12 13 16 weak expression: see section 08 10 14 15

lung

weak

regional

weak expression: see section 02 03 04 05 06 07 10 11 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38999
Entity Detected:	Psmd1, proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 ( MGI:1917497)
Sequence:	sense strand is shown >T38999 GCCAGGATCAGAGAAAGACAGTGACCCCATGGAGACGGAGGAGAAGACAGCCAGTGCAGTGGCTGGGAAG ACACCAGATGCGAGTCCAGAGCCCAAGGACCAGACACTGAAAATGATTAAGATTTTAAGTGGTGAAATGG CTATTGAATTACACCTGCAGTTCCTAATACGAAATAATAACACAGACCTCATGATTCTGAAAAACACAAA GGATGCAGTACGAAATTCTGTGTGTCATACAGCAACTGTAATAGCAAACTCATTCATGCACTGTGGTACT ACCAGTGACCAGTTTCTTAGAGATAACTTGGAATGGTTGGCCAGAGCCACCAACTGGGCCAAATTTACTG CAACGGCCAGCTTGGGTGTAATCCATAAGGGCCATGAGAAGGAAGCACTACAGTTAATGGCAACATACCT TCCTAAGGACACTTCTCCAGGATCAGCCTATCAGGAAGGGGGCGGTCTCTATGCATTGGGTCTCATTCAT GCCAATCATGGTGGTGACATAATTGACTATCTCCTTAATCAGCTCAAGAATGCCAGCAATGATATTGTTC GACATGGAGGCAGCCTGGGCCTTGGTTTGGCTGCAATGGGAACTGCACGTCAGGATGTGTATGATTTGCT GAAAACAAACCTGTATCAGGATGATGCTGTGACAGGGGAGGCAGCTGGCCTAGCCTTAGGTTTGGTTATG TTAGGTTCTAAAAATGCCCAGGCGATTGAGGATATGGTTGGCTATGCACAAGAGACTCAACACGAGAAGA TTCTGCGCGGTCTGGCAGTTGGCATTGCGTTAGTGATGTACGGGAGGATGGAGGAGGCTGATGCTCTCAT TGAGTCTCTCTGTCGTGACAAGGACCCAATTCTTAGAAGATCTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 266627. Forward Primer - name:266627_F_cDNA_Psmd1, sequence:GCCAGGATCAGAGAAAGACAGT; Reverse Primer - name:266627_N_SP6_cDNA_Psmd1, sequence:CCAGATCTTCTAAGAATTGGGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18649 same embryo
	EurExpress:euxassay_016545 same experiment
	MGI:4827486 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source