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EMAGE:18695

Pkd1 polycystic kidney disease 1 homolog ( MGI:97603)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18695 EMAGE:18695 EMAGE:18695 EMAGE:18695 EMAGE:18695
"Pseudo-wholemount" of euxassay_016620. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016620_01 euxassay_016620_02 euxassay_016620_03 euxassay_016620_04
EMAGE:18695 EMAGE:18695 EMAGE:18695 EMAGE:18695 EMAGE:18695
euxassay_016620_05 euxassay_016620_06 euxassay_016620_07 euxassay_016620_08 euxassay_016620_09
EMAGE:18695 EMAGE:18695 EMAGE:18695 EMAGE:18695 EMAGE:18695
euxassay_016620_10 euxassay_016620_11 euxassay_016620_12 euxassay_016620_13 euxassay_016620_14
EMAGE:18695 EMAGE:18695 EMAGE:18695 EMAGE:18695 EMAGE:18695
euxassay_016620_15 euxassay_016620_16 euxassay_016620_17 euxassay_016620_18 euxassay_016620_19
EMAGE:18695 EMAGE:18695 EMAGE:18695 EMAGE:18695 EMAGE:18695
euxassay_016620_20 euxassay_016620_21 euxassay_016620_22 euxassay_016620_23 euxassay_016620_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18695Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18695_wholemount_strong.wlz
18695_wholemount_moderate.wlz
18695_wholemount_weak.wlz
18695_wholemount_possible.wlz
18695_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18695_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45151
Entity Detected:Pkd1, polycystic kidney disease 1 homolog ( MGI:97603)
Sequence:sense strand is shown

>T45151
CACTATTTCCTACACCTTCCGCTCTGTGGGCACCTTCAATATTATTGTCACAGCTGAGAATGAGGTGGGT
TCTGCCCAGGACAGCATCTTCATCTATGTTCTCCAGTTCATTGAGGGGCTGCAGGTGGTAGGGGGTGATA
ACGGCTGCTGCTTCCCTACCAACTACACACTGCAGCTGCAAGCTGCAGTTAGGGATGGCACCAACATCTC
TTATAGCTGGACAGCGCAGCAGGAGGGCAGCCTCATCACACTCTTCGGCAGTGGCAAGTGCTTTTCTCTC
ACTGCACTCAAAGCTAGCACCTACTATGTCCATCTTAGGGCCACTAACATGTTGGGCAGTGCCGCAGCCA
ACCGTACTATAGACTTTGTGGAACCTGTGGAGAGTCTAATCCTATCTGCATCCCCTAATCCAGCTGCTGT
CAACATGAGTCTCACCCTTTGTGCTGAATTGGCTGGTGGCAGTGGTGTTGTTTACACTTGGTACCTGGAG
GAAGGACTGAGTTGGAAGACCTCCATGCCATCTACCACCCACACCTTTGCTACACCTGGCCTGCACTTGG
TCAGAGTCACAGCTGAGAACCAGTTGGGCTCAGTCAATGCCACAGTTGAAGTGGCCATACAGGTGCCTGT
GGGTGGCCTGAGTATCAGAACCAGTGAGCCAGATAGCATCTTTGTGGCAGCTGGCTCCACTCTACCCTTC
TGGGGTCAGCTGGCTGAAGGTACCAATGTGACTTGGTGCTGGACCTTGCCAGGTGGGAGTAAGGACAGCC
AGTACATTGCTGTGCGCTTCTCCACGGCTGGCAGTTTCTCTCTGCAGCTCAATGCTTCCAATGCAGTCAG
TTGGGTCTCAGCCAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 172542. Forward Primer - name:172542_F_Pkd1, sequence:CACTATTTCCTACACCTTCCGC; Reverse Primer - name:172542_R_SP6_Pkd1, sequence:ATGGCTGAGACCCAACTGACT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18697 same embryo
 EMAGE:18698 same embryo
 EMAGE:18699 same embryo
 EMAGE:18694 same embryo
 EMAGE:18696 same embryo
 EurExpress:euxassay_016620 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS