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EMAGE:18742

Map3k13 mitogen-activated protein kinase kinase kinase 13 ( MGI:2444243)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18742 EMAGE:18742 EMAGE:18742 EMAGE:18742 EMAGE:18742
"Pseudo-wholemount" of euxassay_014094. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014094_01 euxassay_014094_02 euxassay_014094_03 euxassay_014094_04
EMAGE:18742 EMAGE:18742 EMAGE:18742 EMAGE:18742 EMAGE:18742
euxassay_014094_05 euxassay_014094_06 euxassay_014094_07 euxassay_014094_08 euxassay_014094_09
EMAGE:18742 EMAGE:18742 EMAGE:18742 EMAGE:18742 EMAGE:18742
euxassay_014094_10 euxassay_014094_11 euxassay_014094_12 euxassay_014094_13 euxassay_014094_14
EMAGE:18742 EMAGE:18742 EMAGE:18742 EMAGE:18742 EMAGE:18742
euxassay_014094_15 euxassay_014094_16 euxassay_014094_17 euxassay_014094_18 euxassay_014094_19
EMAGE:18742 EMAGE:18742 EMAGE:18742 EMAGE:18742 EMAGE:18742
euxassay_014094_20 euxassay_014094_21 euxassay_014094_22 euxassay_014094_23 euxassay_014094_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18742Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18742_wholemount_strong.wlz
18742_wholemount_moderate.wlz
18742_wholemount_weak.wlz
18742_wholemount_possible.wlz
18742_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18742_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon lateral wall mantle layer
weak weak
regionalweak expression: see section 13 14
midbrain mantle layer
weak weak
regionalweak expression: see section 09 10 11 13 14 15 16 17 18
dorsal grey horn
weak weak
regionalweak expression: see section 10 11 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T40242
Entity Detected:Map3k13, mitogen-activated protein kinase kinase kinase 13 ( MGI:2444243)
Sequence:sense strand is shown

>T40242
GGAGGGACTCAGAGCAGTCTTAACGTTTGATCCCTCCTTTGTTTACACTTTATGTTGAGACATTGATTTT
AAAATTCAGTCTCTAATTTTTAGCTTATAGGAAAGATGAAGGAACTTGTACTAAGAAAGCAGTATTGTTT
TGTTAAATTATTCCATTTTCTATGAACTGTAGCAGGACTACACAGAAGAGCTTAAGTTATGGCATTATTA
TTCTTGTTTTTATTTTATATTGTCATAATTTTCTGTTGACCAGAAGGTGTGGCTGACGATGTAGCACAAC
GGCTGTTTATTGCTAACCGTGAATTATTATGGACTTCTGTGGTCCTAAGTGAAGGCTGGGGATGGAGCTG
CCTTTTGTCACTCCCAGCATTCCTCAGTGTTTTCCCTGGTCTCCTGACTACTGTAGCAATGGTCTGAGGA
GGTGTGCTCTGCTCTGACACCCAACGGGTACACTGTGATTCTTTTTTACTTGATTGTAAAAGCTCCTGAA
TATGCAGGTGACCAAGTAGTTTAAAGGCTTCAAAAGTTATAAACTTTCTAAAGTGTTAGAATAAAATCAT
GGGACTGTAAGAGAGGCAAAAAGAGATTGAACGGTCCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 88146. Forward Primer - name:088146_F_cDNA_1300002E11Rik, sequence:GGAGGGACTCAGAGCAGTCTTA; Reverse Primer - name:088146_N_SP6_cDNA_1300002E11Rik, sequence:CTGGACCGTTCAATCTCTTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18737 same embryo
 EMAGE:18738 same embryo
 EMAGE:18741 same embryo
 EMAGE:18739 same embryo
 EMAGE:18740 same embryo
 EurExpress:euxassay_014094 same experiment
 MGI:4826080 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS