EMAGE mouse gene expression spatial database

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EMAGE:18744

Lrrc34 leucine rich repeat containing 34 ( MGI:1919077)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014093. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014093_01	euxassay_014093_02	euxassay_014093_03	euxassay_014093_04

euxassay_014093_05	euxassay_014093_06	euxassay_014093_07	euxassay_014093_08	euxassay_014093_09

euxassay_014093_10	euxassay_014093_11	euxassay_014093_12	euxassay_014093_13	euxassay_014093_14

euxassay_014093_15	euxassay_014093_16	euxassay_014093_17	euxassay_014093_18	euxassay_014093_19

euxassay_014093_20	euxassay_014093_21	euxassay_014093_22	euxassay_014093_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18744_wholemount_strong.wlz

18744_wholemount_moderate.wlz

18744_wholemount_weak.wlz

18744_wholemount_possible.wlz

18744_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18744_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

weak

regional

weak expression: see section 06 07 08 10 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40243
Entity Detected:	Lrrc34, leucine rich repeat containing 34 ( MGI:1919077)
Sequence:	sense strand is shown >T40243 ATGATATTGGACCTGAAGGTGGAGAATTGATTGCTAAAGCACTACATAAAAACAAAACTCTTAAGTATTT GAGAATGACGGGAAATAAGATTGAGAACACAGGAGGCATGCTTTTTGCTGCAATGCTGCAGATGAACTCA AGCCCCTGGAGAAACTGGATCTGGGCGACTGGACTGCAATGTGTGATCGCATTTTCTACGGTACTAACTC AAAACCAAGCAATCAAGGGAATAAACCTGAACCGGCCTATACTGTACGGAGAACAGGAAGAGTCTACTGT CCACATTGGCCACATGTTGAAAGAAAATCATGTCCTTGTTGAGCTACATATGTGCAAACATGGCATGAAA AATTATGGTTTACAGCAGTTATGCAATGCACTGTATTTGAACAGTAGCCTACGCTACCTTGATGTCAGCT GCAATAAAATAACTCGTGATGGAATGGTGTTTTTGGCTGATGTCCTAAAAAGCAATACTACTCTGGAAGT ACTAGATCTTTCTTTTAACAGAATAGAAACTGCAGGCGCAAAATTGTCTGTAGTCAGCAACAAGATAGAA GGAGAGGGGCTTGTTGCACTTTCCCAGTCAATGAAAACCAACCTCGTACTGTCCAATATCTACATTTGGG GAAACAAATTTGATGAGGATACCTGTGTGGCATATTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 77162. Forward Primer - name:077162_F_cDNA_1700007J06Rik, sequence:ATGATATTGGACCTGAAGGTGG; Reverse Primer - name:077162_N_SP6_cDNA_1700007J06Rik, sequence:GAATATGCCACACAGGTATCCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18748 same embryo
	EMAGE:18747 same embryo
	EMAGE:18745 same embryo
	EMAGE:18746 same embryo
	EMAGE:18743 same embryo
	EurExpress:euxassay_014093 same experiment
	MGI:4825992 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source