EMAGE mouse gene expression spatial database

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EMAGE:18747

Wscd2 WSC domain containing 2 ( MGI:2445030)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014115. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014115_01	euxassay_014115_02	euxassay_014115_03	euxassay_014115_04

euxassay_014115_05	euxassay_014115_06	euxassay_014115_07	euxassay_014115_08	euxassay_014115_09

euxassay_014115_10	euxassay_014115_11	euxassay_014115_12	euxassay_014115_13	euxassay_014115_14

euxassay_014115_15	euxassay_014115_16	euxassay_014115_17	euxassay_014115_18	euxassay_014115_19

euxassay_014115_20	euxassay_014115_21	euxassay_014115_22	euxassay_014115_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18747_wholemount_strong.wlz

18747_wholemount_moderate.wlz

18747_wholemount_weak.wlz

18747_wholemount_possible.wlz

18747_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18747_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21 22 23

humerus

moderate

regional

moderate expression: see section 01 02 03 04 20 21 22 23

forelimb digit 1 phalanx

moderate

regional

moderate expression: see section 20 22 23

forelimb digit 2 phalanx

moderate

regional

moderate expression: see section 20 22 23

forelimb digit 3 phalanx

moderate

regional

moderate expression: see section 20 21 22 23

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 21

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 21

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 21

tarsus

moderate

regional

moderate expression: see section 20

fibula

moderate

regional

moderate expression: see section 21 22

tibia

moderate

regional

moderate expression: see section 21 22

femur

moderate

regional

moderate expression: see section 05 14 21 22

telencephalon mantle layer

moderate

regional

moderate expression: see section 05 17

otic capsule

moderate

regional

moderate expression: see section 05 06 07 08 15 16 17

utricle

moderate

regional

moderate expression: see section 05 06 07 17 18

axial skeleton

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14

basisphenoid bone

moderate

regional

moderate expression: see section 10 11 12

temporal bone petrous part

moderate

regional

moderate expression: see section 01 02 03 04 05 16 17 18 19 20 21 22

orbito-sphenoid

moderate

regional

moderate expression: see section 05 06 07

viscerocranium

moderate

regional

Expression in the turbinate bone.

clavicle

moderate

regional

moderate expression: see section 06 07 08 15 16

scapula

moderate

regional

moderate expression: see section 04 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39600
Entity Detected:	Wscd2, WSC domain containing 2 ( MGI:2445030)
Sequence:	sense strand is shown >T39600 GGAGTATGACCCTTACACAGCCGAGATGCGCAGGACCATTGCCGCCTACATCCGAATGGTGGACACGGCA CTACGTAGCCGCAACCTCACTGGGGTGCCTGATGCCTATGGCCCAAGATGATGGGCAGCCAGGGAGGGAC CCAGCATCCGGTGCCCCTTGGCTTCAAAGAGCTGGCTTCCCTCTGGCACAAAGACAGCCCCAGGCTTCTG CCTGCCTTCAGAAAGGCCCCTCTGAGGCCTCAGGAGGAGGAGATATCTAGACACCTTTCCCTGGAGAGTA GGCTGCCTGGTTTGGAGTATCCCAGACCCTGCTATTGGTGGTGGGAGAAGGGAGAGGCTATCTGGCAGAG GTTGGGAGGGTCTGGCTGTGGCAGCTGATGGACATTTTGCAAGCAATGACCCTCTTCTGACTGTGTGGGC CCCTGCCTCTTCCCCTGAGCAGGCACCAGCTTTCTGATCTCTCTCAGGCCACCTCTGTGTGACAGACAGC TGGCTGGGACCTGTCTCCGTGGCCTTGGGCGTCTCACTTCTGAGGACCATGGAGACTGGTGCTCTGGCAT TACAGTGGGAACCGCCCCAGGCTAAAGCACATAAGAGGCCCAAGCCGGGTGTTGCCCCCCCTCCAGGACA GCAACCCTGTCTTGTCTGCTCGTGCTGTCGTGTCTTGGGGATGAGGGTGACGTTGGGGTTCTTGATCCTT CTTCCAAAGTCCCTTAGGTACCAAAAACCAACCCAACAGGTTGGCCATGTGCCACATACGGAAGCCCAGA GGACCCAACATTGCAGGGTGGGACCTGAACTGGCTATCTTACACTGCCTGGATTGATCCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 269762. Forward Primer - name:269762_F_cDNA_4933413A10Rik, sequence:GGAGTATGACCCTTACACAGCC; Reverse Primer - name:269762_N_SP6_cDNA_4933413A10Rik, sequence:GGGATCAATCCAGGCAGTGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18748 same embryo
	EMAGE:18744 same embryo
	EMAGE:18745 same embryo
	EMAGE:18746 same embryo
	EMAGE:18743 same embryo
	EurExpress:euxassay_014115 same experiment
	MGI:4829233 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source