Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18793

Rasal2 RAS protein activator like 2 ( MGI:2443881)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18793 EMAGE:18793 EMAGE:18793 EMAGE:18793 EMAGE:18793
"Pseudo-wholemount" of euxassay_014140. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014140_01 euxassay_014140_02 euxassay_014140_03 euxassay_014140_04
EMAGE:18793 EMAGE:18793 EMAGE:18793 EMAGE:18793 EMAGE:18793
euxassay_014140_05 euxassay_014140_06 euxassay_014140_07 euxassay_014140_08 euxassay_014140_09
EMAGE:18793 EMAGE:18793 EMAGE:18793 EMAGE:18793 EMAGE:18793
euxassay_014140_10 euxassay_014140_11 euxassay_014140_12 euxassay_014140_13 euxassay_014140_14
EMAGE:18793 EMAGE:18793 EMAGE:18793 EMAGE:18793 EMAGE:18793
euxassay_014140_15 euxassay_014140_16 euxassay_014140_17 euxassay_014140_18 euxassay_014140_19
EMAGE:18793 EMAGE:18793 EMAGE:18793 EMAGE:18793
euxassay_014140_20 euxassay_014140_21 euxassay_014140_22 euxassay_014140_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18793Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18793_wholemount_strong.wlz
18793_wholemount_moderate.wlz
18793_wholemount_weak.wlz
18793_wholemount_possible.wlz
18793_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18793_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
brain
weak weak
regionalweak expression: see section 01
diencephalon lateral wall mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 13 14 16 17 weak expression: see section 15
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 10 11 14 weak expression: see section 12 13
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 10 11 16 17 weak expression: see section 02 03 04 05 12 13 14 15
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 14 16 17 19 21 22 weak expression: see section 12 15 20 23
medulla oblongata alar plate mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 10 13 17 weak expression: see section 06 07 11 12 14 15 16
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 08 09 10 13 17 weak expression: see section 06 07 11 12 14 15 16
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 13 14 15 16 17 weak expression: see section 11 18 19 20
rest of cerebellum marginal layer
moderate moderate
regionalmoderate expression: see section 10 weak expression: see section 12
pons mantle layer
moderate moderate
regionalmoderate expression: see section 08 10 11 13 17 weak expression: see section 05 06 07 12 14 15 16
pons marginal layer
moderate moderate
regionalmoderate expression: see section 09
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 16 17 weak expression: see section 15
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 13 14 16 weak expression: see section 15
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 18 19 20
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 06 17
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 07
spinal cord mantle layer
moderate moderate
regionalmoderate expression: see section 09 10 weak expression: see section 11 12 13 14 15 16
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 weak expression: see section 07 08 09 10 16 17
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17
viscerocranium
moderate moderate
regionalExpression in the turbinate bone.
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 12 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39521
Entity Detected:Rasal2, RAS protein activator like 2 ( MGI:2443881)
Sequence:sense strand is shown

>T39521
GAGTACGATAGGACCTTGTCGGAATGGATTTTTTTCAGGGATTCATCTGCCAGTTTAAAAGCCCCACCAT
AGTCCTTACCATTAGGAAAACTTGCAAATACCAAGATACATACAAAACAGTAATTTATGCCACTGATCAA
CCAAGATGCTTCTAACAGACTGTAACCTGCTTTGGAGAGCCGCCGTTTCAGAAGACACAGATAAAAGTGG
TTGACTGTACCAAAACTAGACTAGGAATGATTTCTCTTGAACTCCTGTCAATATTTATTTCCTCCATCTA
TAATGGACAGTTAGATGTGGGATTTTTTGATTTTACTCCAACTTTTTTTAAAGTGCTCTTTAGAGCTGAC
AGATTTGTAAGTACCGTGTCTCTATCTACTGTCTTCATGTTCACTTGTCCACTGCCCTTTCCTCCCAGGC
TGGGAGCGGTCAGCACCAACAGGACTCACTCTCCCCTGGAGAGTGGCCATTCAAGTAGACCACTCATAAG
CCATCTGACTAAATACCCTTTTTCCACACAGAAAAGGGCTAACTATACAGCAGAGCAGGAATCTCTGAAT
ATCCCACTAAACGTTCTCTTCCTATTTGTCAGTTGTATTTTTCCTTAGGATAAAGGTTTTATCCACTATA
TTAGCAAATCTGTGGGCAGGGTAACCCGTCACCACTTAACATGAATTGCTTCCTCTTTCTATTTATCCTC
TCCATAAAATTTTGGAAGCTATCTAAAACTGAGACATGCCCCTTAAAAATG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 269582. Forward Primer - name:269582_F_cDNA_Rasal2, sequence:GAGTACGATAGGACCTTGTCGG; Reverse Primer - name:269582_N_SP6_cDNA_Rasal2, sequence:CATTTTTAAGGGGCATGTCTCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18796 same embryo
 EMAGE:18795 same embryo
 EMAGE:18794 same embryo
 EMAGE:18797 same embryo
 EurExpress:euxassay_014140 same experiment
 MGI:4827623 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS