EMAGE mouse gene expression spatial database

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EMAGE:18830

Apold1 apolipoprotein L domain containing 1 ( MGI:2685921)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014175. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014175_01	euxassay_014175_02	euxassay_014175_03	euxassay_014175_04

euxassay_014175_05	euxassay_014175_06	euxassay_014175_07	euxassay_014175_08	euxassay_014175_09

euxassay_014175_10	euxassay_014175_11	euxassay_014175_12	euxassay_014175_13	euxassay_014175_14

euxassay_014175_15	euxassay_014175_16	euxassay_014175_17	euxassay_014175_18	euxassay_014175_19

euxassay_014175_20	euxassay_014175_21	euxassay_014175_22	euxassay_014175_23	euxassay_014175_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18830_wholemount_strong.wlz

18830_wholemount_moderate.wlz

18830_wholemount_weak.wlz

18830_wholemount_possible.wlz

18830_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18830_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart ventricle

strong

spotted

strong expression: see section 06 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40560
Entity Detected:	Apold1, apolipoprotein L domain containing 1 ( MGI:2685921)
Sequence:	sense strand is shown >T40560 GACTTTTCGCTTGTGTAATCCCAACTGAGTATGTGATCCTTTTTGTCTTTGTAGGGATTGTAGACCCAGC CTGGGCCGCGGAGCTGACTTGCTCTGCTCTTCCCACCCCAGTTTATCCACTCATAGACCAAATCTGACTA TTGCATACTTTCCCAGCATGGCTTCAAATTCACACCTCTAGGTACCCTGAGAGGGAAAGCCACCGGAAGA GTCACTTTAATCGCAACATACTCACCCGCCTTCACTTCTGTAAAGAAGCTGGGGGAGGGGTGAGATGCAG TCCCTGACAATTTTAAATAACTGACCACAAAGAGGAGAGCAACGGCCTTTGAGTTAAACAGTCTTGGACT GATCACATGCTCTGTCCCCCACCGTCGTGTGACTTAGCCACCTTCTCTTGCTGAGACTTGGCTTGTTCAG TGACACAGATGGTGGTAAGAACACACCCTGTTGTGAAGCCCTGAGATAAGAAAGGACCTTCAGAGTCACT CTCAGAAAGTCCAGGAAAGACTGGCCCAGCAGCCCAAGAAAGACTATGCTGTGTGCTTGTAAAGCCAGTT ACTCTGTCCCCTGTAAACTAGAAGACAGAGCAGCACAGACCCTTTAGAGGGCAGTGTTGCCCATTCCATG TAGAGACTGGTACAGAGGTAATGTGAAGTTACCAGCAAAAACAAACAAAATCAAAAACAAAAAATAAAAA CAAAACAAACAAACAAATAAACCCCCAAACCTCTTTATCCTCTGAAAACTCCAGACACCATATC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 162202. Forward Primer - name:162202_F_cDNA_mCG52066.3, sequence:GACTTTTCGCTTGTGTAATCCC; Reverse Primer - name:162202_N_SP6_cDNA_mCG52066.3, sequence:GATATGGTGTCTGGAGTTTTCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18826 same embryo
	EMAGE:18825 same embryo
	EMAGE:18828 same embryo
	EMAGE:18829 same embryo
	EMAGE:18827 same embryo
	EurExpress:euxassay_014175 same experiment
	MGI:4823195 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source