Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18876

Nr4a3 nuclear receptor subfamily 4, group A, member 3 ( MGI:1352457)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18876 EMAGE:18876 EMAGE:18876 EMAGE:18876 EMAGE:18876
"Pseudo-wholemount" of euxassay_014226. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014226_01 euxassay_014226_02 euxassay_014226_03 euxassay_014226_04
EMAGE:18876 EMAGE:18876 EMAGE:18876 EMAGE:18876 EMAGE:18876
euxassay_014226_05 euxassay_014226_06 euxassay_014226_07 euxassay_014226_08 euxassay_014226_09
EMAGE:18876 EMAGE:18876 EMAGE:18876 EMAGE:18876 EMAGE:18876
euxassay_014226_10 euxassay_014226_11 euxassay_014226_12 euxassay_014226_13 euxassay_014226_14
EMAGE:18876 EMAGE:18876 EMAGE:18876 EMAGE:18876 EMAGE:18876
euxassay_014226_15 euxassay_014226_16 euxassay_014226_17 euxassay_014226_18 euxassay_014226_19
EMAGE:18876 EMAGE:18876 EMAGE:18876
euxassay_014226_20 euxassay_014226_21 euxassay_014226_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18876Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18876_wholemount_moderate.wlz
18876_wholemount_weak.wlz
18876_wholemount_possible.wlz
18876_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18876_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cerebral cortex marginal layer
weak weak
regionalweak expression: see section 02 03 04 05 06 07 09 10 11 12 14 15 16 17 18 19 20 21 22
metencephalon floor plate
moderate moderate
regionalmoderate expression: see section 09 10 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T39426
Entity Detected:Nr4a3, nuclear receptor subfamily 4, group A, member 3 ( MGI:1352457)
Sequence:sense strand is shown

>T39426
TTTGAACACCTTCCACACTCACTGTCCTTCCTTCAGGGGAAAAGCAGCTCCCGTAGAAAGCAAAGACTTT
TTTTTCTTTTCCTGGCACCTTTCCTTACAACCTAAAGCCAGAAACCTTGCAGAGTATCGTGTTGGGGTTG
TGTTTTATATTTAGGCTTTGGTGGGTGGGTTGGGAGGGGGTAAATAGTTCATGAGGCTTTTCTAAGAAAT
TGCTGACAAAGCACTTTTGGATGATGCTATCCCAGCAGTGGGGTGGGGAGAAAGGATAATATAACTGTTT
TGTTTTTGTTTTTGTTTTTGTTTTTAAAAAAAAACTCTTTCTGGGGAATATGATTATGGTTGCTTTGTAT
TTAAAAATAAGAACAGCCAAGGGCTGTTCACCAGGGTAGGGCTGTGTCCTACGACGGATCCCTTTAGGAT
ATAC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 158827. Forward Primer - name:158827_F_cDNA_Mm.96708, sequence:TTTGAACACCTTCCACACTCAC; Reverse Primer - name:158827_N_SP6_cDNA_Mm.96708, sequence:GTATATCCTAAAGGGATCCGTCG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18875 same embryo
 EMAGE:18873 same embryo
 EMAGE:18874 same embryo
 EMAGE:18872 same embryo
 EurExpress:euxassay_014226 same experiment
 MGI:4826786 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS