EMAGE mouse gene expression spatial database

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EMAGE:18893

Gm7873 predicted gene 7873 ( MGI:3647803)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014162. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014162_01	euxassay_014162_02	euxassay_014162_03	euxassay_014162_04

euxassay_014162_05	euxassay_014162_06	euxassay_014162_07	euxassay_014162_08	euxassay_014162_09

euxassay_014162_10	euxassay_014162_11	euxassay_014162_12	euxassay_014162_13	euxassay_014162_14

euxassay_014162_15	euxassay_014162_16	euxassay_014162_17	euxassay_014162_18	euxassay_014162_19

euxassay_014162_20	euxassay_014162_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18893_wholemount_strong.wlz

18893_wholemount_moderate.wlz

18893_wholemount_weak.wlz

18893_wholemount_possible.wlz

18893_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18893_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

moderate

regional

moderate expression: see section 01 02 03 16 17 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 05 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 03 04 05 06 14 15 16 17 18 19

vagus x ganglion

moderate

regional

moderate expression: see section 05

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 05 15

spinal cord

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 13 14 15 16

neural retina

moderate

regional

moderate expression: see section 01 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T39944
Entity Detected:	Gm7873, predicted gene 7873 ( MGI:3647803)
Sequence:	sense strand is shown >T39944 CCTCAAAGAGAGTGGCAGGTTAGCCAGGCCAGAGCCTGGACCTGAGGTCAGGAACGGGTCACAGGGACTA ATGGAGGGGCCAAAACCCCTGGGACCCTGTGGACACTCTCACCCCCAGCGTCCTCTGGCCCCAGTTTCAA GCAGCCATGGAGGATACCTGGATCAGCCCTGTCAGGGATTTGTAAGGTGTCCCTGTCTGGTCCCCCTTCA GTCCACTCAGTCATTAGAGTCTTCCAGACCTTTCCCAGCTCCAGGGACAGGGAGTGGGAGACCCAGAGTG GGAGGAGAAGCCCCTGGGATGTTTCTGTCAAGCGAAGGAGAGATCCCACGACGGAGAGCTCGAGAGCCAG CAGGGGCCAGACAAGAAGGGGAGGCTGAGGCAGAGTGGGGCTGGCCCCTACATCCAGGGCATGAGCAGGG GCCACCTAGGCAGACAGGATCCCCCACCTCAGGACCCAGGCCCTGCCCATGCCCAACACTACCTCCTAGA GGAAAGGCCACAGCTTCATCTAGACCAGCTCCCACACAGCTTCAGGGCAGGACACTGGGGTCTGCAGAAC AATCCTTTCTGCAGCTGGAGCAGGAGAACCAGAGTCTGAAAAGGCAGAACCAGGACCTTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 235342. Forward Primer - name:164497_F_cDNA_LOC433005, sequence:CCTCAAAGAGAGTGGCAGGTTA; Reverse Primer - name:164497_N_SP6_cDNA_LOC433005, sequence:GAAGGTCCTGGTTCTGCCTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18890 same embryo
	EMAGE:18894 same embryo
	EMAGE:18895 same embryo
	EMAGE:18891 same embryo
	EMAGE:18892 same embryo
	EurExpress:euxassay_014162 same experiment
	MGI:4825119 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source