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EMAGE:189

Fgf4 fibroblast growth factor 4 ( MGI:95518)
TS10 (7.25 dpc)
in situ hybridisation

Data Images
EMAGE:189 EMAGE:189
Fig 3C Niswander et al, 1992 [PMID:1618140] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright. Fig 3B Niswander et al, 1992 [PMID:1618140] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Corresponding bright and darkfield images are shown below xe - extraembryonic ectoderm, xm - extraembryonic mesoderm, ps - primitive streak, ec - embryonic ectoderm, pe - parietal endoderm, ve - visceral endoderm, * - proamniotic cavity
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
embryo ectoderm
detected detected
regionalFgf-4 RNA is limited to cells adjacent to positive regions of the primitive streak
embryo mesoderm
detected detected
regionalFgf-4 RNA is not observed as cells move laterally around the embryo, except in those mesodermal cells emerging from the distal (rostral) regions of the primitive streak where expression continues within 4-6 cell diameters of the streak (data not shown).
definitive endoderm
not detected not detected
extraembryonic component
not detected not detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17265
Entity Detected:Fgf4, fibroblast growth factor 4 ( MGI:95518)
Notes:Two Fgf4 probes were used by Niswander et al, 1992 [PMID:1618140] , each giving "identical results": the"full length coding sequence (see Hebert et al, 1990 [PMID:2318343] " and "the 3' untranslated region from nt 3630-3819 (see Brookes et al, 1989 [PMID:2740210] ". Unable to ascertain which was used here.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:Swiss Webster
Age:7.25 dpc
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Niswander et al, 1992 [PMID:1618140] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1618140] Niswander L, Martin GR 1992 Fgf-4 expression during gastrulation, myogenesis, limb and tooth development in the mouse. Development (114):755-68
 [ doi:10.1016/0012-1606(90)90211-Z] [ PMID:2318343] H�bert JM, Basilico C, Goldfarb M, Haub O, Martin GR 1990 Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis. Dev Biol (138):454-63
 [ doi:10.1093/nar/17.11.4037] [ PMID:2740210] Brookes S, Smith R, Thurlow J, Dickson C, Peters G 1989 The mouse homologue of hst/k-FGF: sequence, genome organization and location relative to int-2. Nucleic Acids Res (17):4037-45
Links:MGI:1331200 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI