Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:18921

B3galnt2 UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 2 ( MGI:2145517)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18921 EMAGE:18921 EMAGE:18921 EMAGE:18921 EMAGE:18921
"Pseudo-wholemount" of euxassay_015892. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015892_01 euxassay_015892_02 euxassay_015892_03 euxassay_015892_04
EMAGE:18921 EMAGE:18921 EMAGE:18921 EMAGE:18921 EMAGE:18921
euxassay_015892_05 euxassay_015892_06 euxassay_015892_07 euxassay_015892_08 euxassay_015892_09
EMAGE:18921 EMAGE:18921 EMAGE:18921 EMAGE:18921 EMAGE:18921
euxassay_015892_10 euxassay_015892_11 euxassay_015892_12 euxassay_015892_13 euxassay_015892_14
EMAGE:18921 EMAGE:18921 EMAGE:18921 EMAGE:18921 EMAGE:18921
euxassay_015892_15 euxassay_015892_16 euxassay_015892_17 euxassay_015892_18 euxassay_015892_19
EMAGE:18921 EMAGE:18921 EMAGE:18921 EMAGE:18921 EMAGE:18921
euxassay_015892_20 euxassay_015892_21 euxassay_015892_22 euxassay_015892_23 euxassay_015892_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18921Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18921_wholemount_strong.wlz
18921_wholemount_moderate.wlz
18921_wholemount_weak.wlz
18921_wholemount_possible.wlz
18921_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18921_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
submandibular gland primordium
weak weak
regionalweak expression: see section 07 08 09 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63207
Entity Detected:B3galnt2, UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 2 ( MGI:2145517)
Sequence:sense strand is shown

>T63207
CTTGGTACTGAGACACACGCTCGCTAAACTGTTTTTGTGAGTAGAGTCAAAGTAAACACCTCAGGGTTCA
GTGTCACAGGGAGGTGTAGGGGAAGGCACTACTCTGCCTTGCCTGATATGTTTACCTAAGCTTGCACAGG
AGGTGGTGAGACGTGCGAGACAGGCTCAGTGTACGCATAGCATAGAGAAAGTATGTTACAGGTCTGAAGA
TGTGGTTCCTCAGCTTAAAGGACATTTCTCAGAATGACCAACCAAATCACTGTAGTGAACTTTTTAAAAG
TTAAGTAGCCCACAGCAGATGTTAACTGTGCTCAGATTTAGACTACTGTTTCTACTCCATGTTTACATCT
TCACATAGTGAATGCAGTAAGGTGTGAAAACAGAAAGGGCAGAGAAATCAATAGTAATCAAAGCCTTTAT
TATTCTATTAATATACAACCAAATGGTCTTTTCCCAACAAAACATAACATCATTTGTGAGGGCAAAGGTT
TTGTTTCTGGTGTATACATTCCAGTCGTTAAACTCTTACGGTTGGACTGTTACCATCGCACTAGTAGGCA
ATCTCCATTTTCCACAGAATAAAACTGTAATGGCTGTAGGTCATTTTCTAGTTCAATTTCTCTGCCCGGC
ATCTAAAATTAAAATCAAGTAGGGAGCAGTAAGACCGATCTGATGGTGCATGCTATCCAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94064. Forward Primer - name:094064_F_cDNA_D230016N13Rik, sequence:CTTGGTACTGAGACACACGCTC; Reverse Primer - name:094064_N_SP6_cDNA_D230016N13Rik, sequence:ATGGATAGCATGCACCATCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18922 same embryo
 EMAGE:18925 same embryo
 EMAGE:18924 same embryo
 EMAGE:18923 same embryo
 EMAGE:18920 same embryo
 EurExpress:euxassay_015892 same experiment
 MGI:4823371 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS