EMAGE mouse gene expression spatial database

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EMAGE:18934

Pknox2 Pbx/knotted 1 homeobox 2 ( MGI:2445415)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014230. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014230_01	euxassay_014230_02	euxassay_014230_03	euxassay_014230_04

euxassay_014230_05	euxassay_014230_06	euxassay_014230_07	euxassay_014230_08	euxassay_014230_09

euxassay_014230_10	euxassay_014230_11	euxassay_014230_12	euxassay_014230_13	euxassay_014230_14

euxassay_014230_15	euxassay_014230_16	euxassay_014230_17	euxassay_014230_18	euxassay_014230_19

euxassay_014230_20	euxassay_014230_21	euxassay_014230_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

18934_wholemount_moderate.wlz

18934_wholemount_weak.wlz

18934_wholemount_possible.wlz

18934_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:18934_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

moderate

regional

moderate expression: see section 13 14 weak expression: see section 09 10 11 12

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 09 weak expression: see section 07 08 10 11 12 13 14

dorsal grey horn

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 13 14

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 14 15 16

tongue muscle

moderate

regional

moderate expression: see section 13 weak expression: see section 11 12 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38705
Entity Detected:	Pknox2, Pbx/knotted 1 homeobox 2 ( MGI:2445415)
Sequence:	sense strand is shown >T38705 TTCATATCAGAGTGGAACGTGGACTGCACAGGGCTCCAACTTACCCTACCAGGACCCTGTCTCTTGGCAC AGCAGCCATGGACTATAAGGAGAGGGGGAAGGAAACCAAAGAGACTCCTTCTTCCTCTCTTGGAAATACA TTGTAGGTGGGTCCAACCTGAAGAGGTTAAGAGCAACTCTACATCTGGGTCAGCTCGAGGACCAAGCAGG TAGCAGAACCTGGATCAGGAGCCATATTAAGATGACTTTGGGGATCCCAGCAGTCCCTACGTGATCACAT AGCTCCTGTCACAATAGGACCATAGTGTCTTATTAGATGTTCCTACCCTCAGATGGAGTTCTTCAGAGAC CCCAGCCCCCTCCATAAACCTGTAGGGGTTCTTTCCACTCCACAGGTCTTCGTGGTATCCCTCTGCTCCC AAGCTGGCACTACCAGCTTCTGGCCTCCTCCTTCATTCTATTCTCCAGAGAAGGCAGAAGAAACATTGGA AAGAAGCATTGTCCCAATGGGAAGCCAGGGTCTGAAGAAGGTGCTACTGCTCTTACCTTCAGGACTCAAC ATGGCAGGGGATATAGCGCCCAGCTGATCCACACACACCATGCTTGGCATCTGCTCCTCCTGTCCCCTCC CACCCTGTGGCTGTGAAAGACAGGACTGACCACACGTGTGTTTTCCGTTGGGTTTAATGTGATGGGTGTG CAGTTCCTTCATTTGTAAGTGTGCATTGGCCATCTCCTTCAGTGCTAGGATGTGAATGGCTATCTGGCTC AACCAGAGGGCACTCTTGCGACCTTCTGAGCCTTCTCCTCCTCTACCTGGTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 158467. Forward Primer - name:158467_F_cDNA_Mm.32408, sequence:tTCATATCAGAGTGGAACGTGG; Reverse Primer - name:158467_N_SP6_cDNA_Mm.32408, sequence:AACCAGGTAGAGGAGGAGAAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:18935 same embryo
	EMAGE:18933 same embryo
	EurExpress:euxassay_014230 same experiment
	MGI:4827237 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source