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EMAGE:18961

Cyp2s1 cytochrome P450, family 2, subfamily s, polypeptide 1 ( MGI:1921384)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18961 EMAGE:18961 EMAGE:18961 EMAGE:18961 EMAGE:18961
"Pseudo-wholemount" of euxassay_015876. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015876_01 euxassay_015876_02 euxassay_015876_03 euxassay_015876_04
EMAGE:18961 EMAGE:18961 EMAGE:18961 EMAGE:18961 EMAGE:18961
euxassay_015876_05 euxassay_015876_06 euxassay_015876_07 euxassay_015876_08 euxassay_015876_09
EMAGE:18961 EMAGE:18961 EMAGE:18961 EMAGE:18961 EMAGE:18961
euxassay_015876_10 euxassay_015876_11 euxassay_015876_12 euxassay_015876_13 euxassay_015876_14
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euxassay_015876_15 euxassay_015876_16 euxassay_015876_17 euxassay_015876_18 euxassay_015876_19
EMAGE:18961 EMAGE:18961 EMAGE:18961 EMAGE:18961
euxassay_015876_20 euxassay_015876_21 euxassay_015876_22 euxassay_015876_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18961Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18961_wholemount_strong.wlz
18961_wholemount_moderate.wlz
18961_wholemount_weak.wlz
18961_wholemount_possible.wlz
18961_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18961_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
stomach
weak weak
regionalweak expression: see section 05 06 07 08 09 10
midgut
weak weak
regionalweak expression: see section 07 08 09 10 11 12 13 14 15
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63052
Entity Detected:Cyp2s1, cytochrome P450, family 2, subfamily s, polypeptide 1 ( MGI:1921384)
Sequence:sense strand is shown

>T63052
GAATTCACCGAGAAGAACTTGCTGATGACGGTCACATACCTGCTGTTTGCTGGGACCATGACCATCGGTG
CCACCATCCGCTATGCCCTCCTGCTCCTGCTGAGATACCCTCAAGTCCAGCAGCGCGTCCGGGAGGAGCT
CATACAGGAGCTGGGTCCTGGCAGGGCTCCAAGTCTCAGCGATCGAGTTCGCCTCCCTTACACGGATGCC
GTTTTACACGAGGCACAGCGGCTCCTGGCACTGGTACCCATGGGCATGCCCCACACCATCACGAGGACCA
CTTGCTTCCGAGGGTACACTCTGCCCAAGGGCACTGAGGTCTTCCCTCTGATTGGCTCCATACTGCATGA
CCCTGCGGTTTTCCAGAACCCAGGAGAGTTCCATCCAGGCCGCTTCCTGGACGAAGATGGTCGGTTGAGA
AAACACGAAGCCTTCCTGCCCTACTCCTTAGGTAAGCGAGTCTGCCTGGGAGAAGGCCTGGCTCGGGCGG
AGTTGTGGCTTTTCTTCACTTCCATCTTGCAAGCCTTCTCCCTGGAGACCCCGTGCCCGCCGGGTGACCT
GAGCCTGAAGCCAGCCATCAGTGGACTTTTCAACATCCCCCCGGACTTCCAGCTGCGGGTCTGGCCCACT
GGCGACCAGTCCAGATGAAGGAAGGAGTTTGGAAGGTGGGAGCCCTCTGGGCTGAAAGAGCCTTACTCAG
GGTGTGTGTGAAGCAGGTGTCTCAGAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91519. Forward Primer - name:091519_F_cDNA_Cyp2s1, sequence:GAATTCACCGAGAAGAACTTGC; Reverse Primer - name:091519_N_SP6_cDNA_Cyp2s1, sequence:TTCTGAGACACCTGCTTCACAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:18962 same embryo
 EurExpress:euxassay_015876 same experiment
 MGI:4824175 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS