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EMAGE:18999

Plxnb2 plexin B2 ( MGI:2154239)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:18999 EMAGE:18999 EMAGE:18999 EMAGE:18999 EMAGE:18999
"Pseudo-wholemount" of euxassay_014231. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_014231_01 euxassay_014231_02 euxassay_014231_03 euxassay_014231_04
EMAGE:18999 EMAGE:18999 EMAGE:18999 EMAGE:18999 EMAGE:18999
euxassay_014231_05 euxassay_014231_06 euxassay_014231_07 euxassay_014231_08 euxassay_014231_09
EMAGE:18999 EMAGE:18999 EMAGE:18999 EMAGE:18999 EMAGE:18999
euxassay_014231_10 euxassay_014231_11 euxassay_014231_12 euxassay_014231_13 euxassay_014231_14
EMAGE:18999 EMAGE:18999 EMAGE:18999 EMAGE:18999 EMAGE:18999
euxassay_014231_15 euxassay_014231_16 euxassay_014231_17 euxassay_014231_18 euxassay_014231_19
EMAGE:18999 EMAGE:18999 EMAGE:18999 EMAGE:18999 EMAGE:18999
euxassay_014231_20 euxassay_014231_21 euxassay_014231_22 euxassay_014231_23 euxassay_014231_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:18999Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
18999_wholemount_strong.wlz
18999_wholemount_moderate.wlz
18999_wholemount_weak.wlz
18999_wholemount_possible.wlz
18999_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:18999_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
medulla oblongata part of 4th ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 03 04 05 06 07 08 09 15 16 17 18 19 20 weak expression: see section 10 11 12 13 14
hypothalamus ventricular layer
weak weak
regionalweak expression: see section 12 13
diencephalon lateral wall ventricular layer
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 12 13
lateral ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 07 08 09 15 16 17 18 weak expression: see section 10 11 12 13
telencephalon ventricular layer
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
medulla oblongata basal plate ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 08 12
rest of cerebellum marginal layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 weak expression: see section 08 12 13 14 15 16 17 18 19 20
rest of cerebellum ventricular layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 weak expression: see section 15 16 17 18 19 20
pons ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11 12 15 weak expression: see section 06 07 08 13 14
midbrain ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11 weak expression: see section 06 07 08 12 13 14
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 10 11 12 weak expression: see section 13 14
naris
moderate moderate
regionalmoderate expression: see section 15 16 18 19
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 10 11 12 13 14 15 16 17 18 19 20
vomeronasal organ
moderate moderate
regionalmoderate expression: see section 14 17
mandible
moderate moderate
regionalmoderate expression: see section 10 11 20 weak expression: see section 19
lower jaw molar
moderate moderate
regionalmoderate expression: see section 09 19 20
upper jaw molar
moderate moderate
regionalmoderate expression: see section 09 10 11 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38752
Entity Detected:Plxnb2, plexin B2 ( MGI:2154239)
Sequence:sense strand is shown

>T38752
CAAAGCAGATGACGTCAAGAAGATAACTGTGGCTGGCCAGAACTGTGCCTTTGAACCAAGAGGGTACTCC
GTATCCACCCGGATTGTGTGTGCAATTGAGGCTTCGGAGATGCCCTTCACAGGAGGCATTGAGGTGGATG
TTAATGGAAAACTCGGCCATTCACCGCCACACGTCCAGTTCACTTATCAACAACCCCAGCCTCTCAGTGT
GGAGCCACGACAGGGGCCACAGGCAGGTGGCACCACATTGACCATCAATGGCACTCACCTGGACACAGGC
TCCAAGGAGGATGTGCGGGTGACACTCAATGACGTCCCTTGTGAAGTGACAAAGTTTGGAGCACAGCTAC
AGTGTGTCACAGGTCAACAGTTGGCTCCAGGCCAGGTGACACTAGAAATCTACTATGGGGGCTCCAGAGT
GCCCAGCCCCGGCATCTCTTTCACCTACTGCGAGAACCCCATGATACGAGCCTTTGAGCCATTGAGAAGC
TTTGTCAGTGGTGGCCGGAGCATCAACGTTACTGGCCAGGGCTTCAGCCTCATCCAGAAGTTTGCCATGG
TTGTCATCGCTGAGCCCTTGCGGTCCTGGAGGCGGCGGCGGCGGGAGGCTGGAGCCCTGGAGCGTGTGAC
GGTCGAGGGCATGGAGTACGTGTTCTACAACGACACCAAGGTCGTCTTCTTGTCTCCTGCTGTCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 161347. Forward Primer - name:161347_F_cDNA_mCG140951, sequence:CAAAGCAGATGACGTCAAGAAG; Reverse Primer - name:161347_N_SP6_cDNA_mCG140951, sequence:GGGACAGCAGGAGACAAGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:MGI:4827293 same experiment
 EurExpress:euxassay_014231 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS