EMAGE mouse gene expression spatial database

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EMAGE:19007

Pcdhga10 protocadherin gamma subfamily A, 10 ( MGI:1935227)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002107. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002107_01	euxassay_002107_02	euxassay_002107_03	euxassay_002107_04

euxassay_002107_05	euxassay_002107_06	euxassay_002107_07	euxassay_002107_08	euxassay_002107_09

euxassay_002107_10	euxassay_002107_11	euxassay_002107_12	euxassay_002107_13	euxassay_002107_14

euxassay_002107_15	euxassay_002107_16	euxassay_002107_17	euxassay_002107_18	euxassay_002107_19

euxassay_002107_20	euxassay_002107_21	euxassay_002107_22	euxassay_002107_23	euxassay_002107_24

euxassay_002107_25	euxassay_002107_26	euxassay_002107_27	euxassay_002107_28

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19007_wholemount_strong.wlz

19007_wholemount_moderate.wlz

19007_wholemount_weak.wlz

19007_wholemount_possible.wlz

19007_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19007_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5492
Entity Detected:	Pcdhga10, protocadherin gamma subfamily A, 10 ( MGI:1935227)
Sequence:	sense strand is shown >T5492 CCACGCGTCCGGCAACGAAAATTACTCCGCACTTTAAGGTGCTTCCGCTGCGGGAGACCCAAGGGGTCAC AGAAAGAAGAAAAGAACCGGGTGAAACACACTGGGCGCCTGGCGGATCGGGAAAAGCTTTGAGAGAATTC CGTAGTCAGAAGACAATGGCCGCTCAAAGGAATCTCTCAGAGCGCACCAAACTGGTCCTCCTTTTCTTAT GGATGCCCTGGGAATCCGCAGCTCGGCAGATCCGCTATTCAGTTCCTGAGGAACTAGAGAAAGGCTCTTT TGTGGGCAACATCGCCAAGGACCTGGGGCTGGAGCCCCGCCAGCTGGCGGAACGCGGGGTCCGCATCATC TCCAGAGGTAGGTCTCAGCTTTTCTCTCTGAACCCGCGAGGCGGCAGCTTAGTCACCGCGGGCAGGATAG ACCGGGAGGAGCTGTGCGCCCAGAGCGCGCCCTGTCTCGTCAGCTTTAACATCCTTGTTGAAGATAGAGT GAAACTTTTCGGGATAGAAATAGAGGTAACGGATGTCAATGACAATGCGCCAAAATTCCAAGCAGAAACT CTAGATGTAAAAATTAATGAAAATGTCGCCCCAGGAATGCGTTTTCCTCTCCCGGAAGCTACTGATCCGG ATGTGGGAGTGAATTCTCTGCAGAGCTACCAGCTCAGCTCCAATAAACACTTCTCCCTAGTCGTTCAGAG CGGTGCTAAGGGCGTCAAGTACCCGGAGCTAGTGCTGGAACAGGCCCTAGATCGTGAGGAAGAGGCGATT CACCACCTGGTCCTCGTGGCCTCTGACGGAGGAAACCCTCCCAGATCGGGCACTGTCCTCATCACTGTGA CCGTCTTTGATGCAAATGACAATGCTCCAGTCTTCACCTCCACTGAATACCGCGTGAACATTCCGGAGAA CCTGCCTGTGGGCACCCAACTGCTAAAGGTAACCGCCACCGACAAAGACGAAGGTGCCAATGGAGAAGTG ACATATGCATTCCGAAAATCGCTGGACACACAATTGTCCAAATTCCAACTAGACAAACATACCGGAGAAA TAAAAATTTCTGAAAATCTAGATTATGAAGAAATGAGTTTCTATGAAATAGAAATCCAAGCTGAAGATGG AGGGGCCTATCTTGCAACTGCGAAGGTGCTGATTACAGTAGAAGATGTAAATGACAACAGTCCTGAGGTG ACCATCACATCTCTCTTCAGTCCAGTGACAGAAGATTCGCCGGTGGGAACTGTCATAGCCCTACTAAACG TCCATGATTTAGACTCCGGGCAGAATGGAGAGGTAGCCTGTTCCATCTCAGGGAATCTTCCGTTTAAGTT AGAAAAGTCCATTGACAATTACTATAGGCTAGTGACACAAAGAGCCCTTGATCGGGAACAGGTACCCGCT TACAACATCACAGTAACAGCCACTGATGGGGGAAACCCACCTTTGTCAACCAAAGCTGACTTCACTCTGC AAGTGGCAGATATCAACGACAACCCACCTACCTTCTCTCATCCGTCCTACTTTACCTACATCCCAGAGAA CAATCCCAGAGGCGCCTCCATCTTCTCAGTGACTGCCCTCGATCCAGACAGCAAAGAGAACGCCCAGATC GTTTACTCCCTGGCTGAAGACACCATTGAGGGGGCGCCACTTTCTTCCTACATATCCATCAACTCAGACA CTGGCATCCTGTATGCGCTCTGGTCCTTTGACTATGAGCAGTTTCGTGAACTGCAGGTACAGGTGACTGC CAGCGACAGCGGAAAGCCGCCCCTCCACAGTAATGTGTCGTTGACTCTGTTTGTGCTGGATCAAAATGAC AACTCTCCAGAAATCTTGTACCCCACCATGCCCACCGACGGCTCCACTGGGGTAGAGCTTGCTCCCCGCT CAGCAGAGCTGGGCTATCTGGTGACGAAGGTGGTGGCAGTGGACAGAGACTCGGGACAGAACGCCTGGCT GTCCTATCGCCTACTCAAGGCCAGCGAACCAGGGCTCTTCACTGTAGGGCTGCACACGGGTGAGGTGCGC ACAGCCCGAGCCCTGATGGAAAGAGATGCTCTCAAGCAGAGTCTAGTGGTGGCTGTGCAGGACCATGGCC AGCCTCCTCTCTCCGCAACGGTCACTTTGACTGTTGCAGTAGCTGACAGCATCCCTGATGTCCTGGCAGA CTTGGGCAACCTGGAGCCACCACAGGACTCTGAGGCCTCAAACCTCACACTCTACCTAGTAGTGGCAGTG GCAGCTGTTTCTTGCGTCTTTCTCGCCTTTGTCATTGTGTTGTTGGCACTCAAACTCCGGCGCTGGCACA AGTCTCACGTGCTGCAGTCTTCGAGAGATGGATTGGCTGGCGTGCCTGCCTCTCACTATGTGGGCGTGGA CGGGGTGCGCGCTTTCCTGCAGACCTATTCGCACGAGGTTTCCCTCACTGCTGATTCGCGGAAGAGTCAT CTGATCTTCCCGCAGCCTAACTACGCAGACACACTCATCAGCCAGGAGAGCTGTGAGAAAAAAGATCCTT TGTCTTTGTTAGATGATTCCAAGTGTCCTGTAGAAGACGCTCCTTTGGTGCCACAAGCCCCGCCCAACAC TGACTGGCGTTTCTCTCAAGCCCAGAGACCCGGCACGAGCGGATCCCAAAATGGTGATGAAACTGGCACC TGGCCCAACAACCAGTTTGATACAGAGATGCTGCAAGCCATGATCTTGGCCTCTGCCAGCGAAGCTGCTG ATGGGAGCTCTACCCTGGGAGGGGGCGCTGGCACCATGGGTCTGAGCGCTCGATACGGACCCCAGTTTAC CCTGCAGCACGTGCCTGACTACCGCCAGAACGTGTACATCCCTGGCAGCAATGCCACGCTGACCAATGCC GCTGGCAAACGAGATGGCAAGGCTCCAGCAGGTGGTAATGGCAACAAGAAGAAATCGGGCAAGAAAGAGA AGAAGTAATATGGAGGCCAGGCCTTGAACCACAAGGCAGCCTCCCTCCCCAGCCAGTCCAGCTCGTCCTT ACTTGTACCCAGGCCTCAGAATTTCAGGGCTCACCCCAGGATACTGGTAGGAGCCAGGGCCATGCTCCCC GTTGGGAAACAGAAACACGTGCCCAAGCCAACACCCCCTCTCTATACCCTAGGGAGTTGAATATGCAAAG AGAGTTCTGCTGGGACCCCCCTATTCAATCAGTGATTGTACCCACATGGGTAGCAGGGTTTGTGTGTGTG GACATACACACACACACACACACACACACACACAAACCATGCCACACTCCCTTAGTTACAGCTGAACTCC TCCATCTTCCAACCCAGGCAGGCTTATCCATCTCCTGCCGCCCTCTCCCCACCCCACCCACTCCTTCAGT CCCTCTCTTTGAGCAAGGTAGCTGGGCTGTTGGGGTGACCGGTGAGCTAAGCCGGCTCCTAGTTCTGAAC GGTAGGGAGAGTATTACAATCTTTTGGCCTCTCCTTCAGTTCAGCTTTCCCCCAAAGCATGGTTTGGTAC CAGTCTTCTCTTTTCCTTCCAGAGCCTGAGACCAATGCTTAAGTTTTGGGGGAACAGGCACCCTCCCTCT GGTACTGATGATGCTTGCTGGATTTTGGGAAGGCATTTTGCTACTAAGCCTCTTTTCAATGCCTGGGGAC AACTAGTCTTTTGTTTTTCATTGTTTGATGTTCCCACTGCATGCTGTGACTTCCCTACACCTCAAAGAAG AGACTCCTTTGCATGTCTGGAGACAGTAGGGGGTGGTAAACTAAAGGGAAGTTGAGAGTGTATACTGCTG GGAGTGGGGCAGTGGACAAAACAGCCTGTTAATAGGGTCTTGCAAGGGGCTCTGTATGTACCCAGGGGAC TGGCTCAATCCTCACATTCTAGCCATAGACAAACACCAGGCCAGACCCCTCCATTCTGGTTCAGCCTGGG CAGCTTGGGCTGAGCCACCAGGACCAATGGATTTAAGCTGACATTTCAGTCCAAGACGACGACTTCTAAG TGAGTTTAAGACCAGAGAGGAAAGAGGGGCCTCTGTGGGTGCTGGGTACTCCAGAGGTGCCCTTGGTGGG AGGACCAGTGGTCTTAGCAGGAAGGGGGGCCCAGCAAGGTCATTCTTGGACCCTGGGTCTAGTCCAGTAG CTAGAAAAAGGGACCAAGTGGCCATAAAGTCCCAGCCAATGATGGGGCTTTTCCAGTGGGCCCCTGTAGA CCTCAAGCCCCTGGCCTCCACCTTACCAGGTGCCATTTCTTCTCAGAAGGCCACTGCCCAGGCCCCCAGG CCGCCCCCTTGTGGCCAGAGAACGGTTAAAGCCCCCCAGTGCCTCCTTGTGCATAGAACTTCCTCTGCCC TAGCCCTTACAGTAGTGTAGAAGATCCCCCTCCCCCGCTGGTGTAGAATAGCCAATAGTATAGTGTGGTG TGCTTTTACGTGATGGCGAGTGGGCAGCGGGCGGTGGGCTGTACACAGCCGTCTGTCCTTTGAATCTCAA TCTGCCTGCGCGGCCCGTGCTGTGTTTTGTGCTGTGTCCACACGCTGCGGCGACCCCTCCCTGTACTGAC TTCTCTATAAAAAGCTTCTCTTCGCATAGTCACGTAGCTCCTGCCCATCCCCCTTCCGATATCTCTCGCA AGTTTTATACTCTAATATTTATATGGCTTTTTTTCTTCGAAAATTAAAAATAAAAAAATGGTTTCTTCTG AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5400956 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5400956 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19006 same embryo
	EurExpress:euxassay_002107 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source