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EMAGE:19016

Atxn3 ataxin 3 ( MGI:1099442)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19016 EMAGE:19016 EMAGE:19016 EMAGE:19016 EMAGE:19016
"Pseudo-wholemount" of euxassay_015931. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_015931_01 euxassay_015931_02 euxassay_015931_03 euxassay_015931_04
EMAGE:19016 EMAGE:19016 EMAGE:19016 EMAGE:19016 EMAGE:19016
euxassay_015931_05 euxassay_015931_06 euxassay_015931_07 euxassay_015931_08 euxassay_015931_09
EMAGE:19016 EMAGE:19016 EMAGE:19016 EMAGE:19016 EMAGE:19016
euxassay_015931_10 euxassay_015931_11 euxassay_015931_12 euxassay_015931_13 euxassay_015931_14
EMAGE:19016 EMAGE:19016 EMAGE:19016 EMAGE:19016 EMAGE:19016
euxassay_015931_15 euxassay_015931_16 euxassay_015931_17 euxassay_015931_18 euxassay_015931_19
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euxassay_015931_20 euxassay_015931_21 euxassay_015931_22 euxassay_015931_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19016Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19016_wholemount_strong.wlz
19016_wholemount_moderate.wlz
19016_wholemount_weak.wlz
19016_wholemount_possible.wlz
19016_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19016_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63045
Entity Detected:Atxn3, ataxin 3 ( MGI:1099442)
Sequence:sense strand is shown

>T63045
GGCAGCTGTAGGGTGTTAGAGTTAGGACTTTGCTTACAGTTGATTGCTATACTGAAGTATGTAACTACTC
CCGTATGCTGCTCACATGTCTAGACATTCATTGGCCCTTTTCCTTTACCCTTTGAAAAATCACATTAAAC
TTTCCATACATTTAGATTGGTGTGCTGCCTGATGAAAAATGAGAAAGCCAATATACACTTCAGCTTTAAC
TTTAGAGTTTATAAAATGTTCATATAACACACCCTACTTAAATCATGTATGAAATACAGCATGTTAGTCT
TTTATTAGAAATGTTTTAAAGAGAAACTGAGCACACACTGAATCTATTTGAGTGAAGCTTTTTAGTTTGT
TATATTTATGGCATTGTCCAGGCAGATCCAAAATCACCATTGAATTGCCATCCTCTTTGGGTTGACCTAG
GCTTACTAAACATTTAGATTTCATTATTAATGAGTAGCCAGTATGAAAAGATGCCAATGCTTATTCTAGC
CACAGTATCCAAAGTGTAGGAGTTCTATGACATTCTAATTCATAACCAGGAGTTGCTGTTTATAGTACTG
CAGGACAGACAGGGGCAAGGGACTCCACTGCCACGGCTGGTCACAGACTCGACTAACTTTTCCATTTGTT
TTTTTACCAATTGGAATACTTAAATGTATTGTATGTAATGAATACTAAACTACTGATAATCATTTTTTTT
AAATCTGTAACTTGTTCTGCCAGATAAAATGCGTACAGCCGTGATGCTAGTGTTATAACCAAGTGTAAGG
ACCCTTTGGAAGGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91659. Forward Primer - name:091659_F_cDNA_Mjd, sequence:GGCAGCTGTAGGGTGTTAGAGT; Reverse Primer - name:091659_N_SP6_cDNA_Mjd, sequence:GCCTTCCAAAGGGTCCTTACA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19014 same embryo
 EMAGE:19015 same embryo
 EMAGE:19019 same embryo
 EMAGE:19018 same embryo
 EMAGE:19017 same embryo
 EurExpress:euxassay_015931 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS