EMAGE mouse gene expression spatial database

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EMAGE:19031

Cpm carboxypeptidase M ( MGI:1917824)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015808. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015808_01	euxassay_015808_02	euxassay_015808_03	euxassay_015808_04

euxassay_015808_05	euxassay_015808_06	euxassay_015808_07	euxassay_015808_08	euxassay_015808_09

euxassay_015808_10	euxassay_015808_11	euxassay_015808_12	euxassay_015808_13	euxassay_015808_14

euxassay_015808_15	euxassay_015808_16	euxassay_015808_17	euxassay_015808_18	euxassay_015808_19

euxassay_015808_20	euxassay_015808_21	euxassay_015808_22	euxassay_015808_23	euxassay_015808_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19031_wholemount_strong.wlz

19031_wholemount_moderate.wlz

19031_wholemount_weak.wlz

19031_wholemount_possible.wlz

19031_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19031_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 10 11 13 14

submandibular gland primordium

strong

regional

strong expression: see section 06 moderate expression: see section 07 08 09 17 18 weak expression: see section 16

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 10 11 12

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 11 14 15 16 17 weak expression: see section 13

stomach

weak

regional

weak expression: see section 04 05 06 07 08

midgut

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14

genital tubercle of male

moderate

regional

moderate expression: see section 13 weak expression: see section 14 15 16

pelvic girdle skeleton

weak

regional

weak expression: see section 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40478
Entity Detected:	Cpm, carboxypeptidase M ( MGI:1917824)
Sequence:	sense strand is shown >T40478 GTTCTGACTGGTTGACCCTTTCCTAAGATTCCGTGAAAGGAAATGAGCTGTTCGCTGAGCTGCTCATGGG CCTAACCGGAGACTTGGTACTGAAACAACTTGCAAGTGCCCGTGTCTTACCAGAGACTGTGCCAGGGCTT TGCACAGGGCCATTGAGCCACAGCTACGTCTTTATTTTGTTACTTTTTGTTCAGACACAGCATTTCACTA AGCTGTGTAGCCTGCACTTGGAACTCATAGTAAGCCAAACTGGCTTTGGATCTGCCTGCCGTAGACGCTG GAGTAGCTATTACAGACCTGGTCCGCTGTGTCCATATTCAGGTTACATTACTTAAATCAGAAATATATAT CAAAACCCAGTGTAAAAATCACTAAAACAGGGTGAGGGGTGTAGCTCAGTGACAGTGTACTTAGCATATG CAAAGGCTTGGGTCTAATCTCCATTAACTACCCCAAGACATACATAACAGGAAATTATCTGTTGCCTCTG CCAGTAGAGAAAGCAGAGGATACTTGGTCTGTACTGTTTGAAGCATATACTACTAAAGTGACCTGNCACG GAATGCTGGTACCAATAACCACGTCCTTTCAAGGAAACTTTCACAAAGTGCCTAGTTCACATGCGTGCCC ATTTGTGATATGAGATGGTAAAATCCTAGCACCCAGGCAGCAGAGGCAGGTGGATCTCTGTGAGTTCAAG GCCAGTCTTTTCTACAGAGTTCTGGGACCGCCAGGCGTACGCAGAAAAACCCCCGTTTCAAACAAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 160157. Forward Primer - name:160157_F_cDNA_Mm.319964, sequence:GTTCTGACTGGTTGACCCTTTC; Reverse Primer - name:160157_N_SP6_cDNA_Mm.319964, sequence:cttgtttgaaacgggggtttt. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19032 same embryo
	EMAGE:19030 same embryo
	EurExpress:euxassay_015808 same experiment
	MGI:4824028 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source