EMAGE mouse gene expression spatial database

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EMAGE:19090

Tnks tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase ( MGI:1341087)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_015951. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_015951_01	euxassay_015951_02	euxassay_015951_03	euxassay_015951_04

euxassay_015951_05	euxassay_015951_06	euxassay_015951_07	euxassay_015951_08	euxassay_015951_09

euxassay_015951_10	euxassay_015951_11	euxassay_015951_12	euxassay_015951_13	euxassay_015951_14

euxassay_015951_15	euxassay_015951_16	euxassay_015951_17	euxassay_015951_18	euxassay_015951_19

euxassay_015951_20	euxassay_015951_21	euxassay_015951_22	euxassay_015951_23	euxassay_015951_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19090_wholemount_strong.wlz

19090_wholemount_moderate.wlz

19090_wholemount_weak.wlz

19090_wholemount_possible.wlz

19090_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19090_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex mantle layer

weak

regional

weak expression: see section 06 07 08 09 10 11 12 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 11 12 13 14 15 17

pons mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 18 19 20

midbrain mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 weak expression: see section 20 21 22

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 weak expression: see section 04 18 19 20 21 22 23

dorsal grey horn

weak

regional

weak expression: see section 13 14

ventral grey horn

moderate

regional

moderate expression: see section 11 15 18 weak expression: see section 12 13 14

dorsal root ganglion

strong

single cell

strong expression: see section 09 10 11 12 13 14 17 18 19

neural retina

weak

regional

weak expression: see section 01 02 03 04 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63467
Entity Detected:	Tnks, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase ( MGI:1341087)
Sequence:	sense strand is shown >T63467 TCAAACAACCGCTGACTACATCCAGTGCATGAAAAAGATCACTTAAAAAATACCGCCCTAAAAAACTGAA GCTGTTTCAGCCAACTCAAGCAATCTGCAGTTAATAGACAACTATGGTTTTCCTGTTTGAAAAAGCCTTA TCTATGGCAGAAACTTTTTAACCTTTTCTAATCTAGCAGTGAAATATGAATCCACTTCTTAATATTTGAA ATGTGTCAGTGAGTGGGCGTGTCTGTTCATTTCATACCTGGTAAGTGTGTTTTATGGGGTATATTTTATA AGTAATATTGTCACATTTAGTGGAGTTGATGTAGCCAGTCAGTTCCAATTTCTCTCATGCAAAGTTCTTC AGCCTCCTGGTCACCTGGTTTGGAGTAACACTGGTGGGATTGCCGAACTTGTGTGGGGGATTCATAGTAT GTAATACTGTGTAGCATTTGAGTGGTGTGGAAGTTGTTTTTAAATCATTTTAAAGTATATACTATGACAG ATGAAACTTAAAACTTGTCGGAAGCTGAAGATTTTATTTCTGTGAGATTCAGTGTGGTCAGGTTGTGTGG CCAATTTTATACTTCTACCAGAATTCTGCAAAATTGAGGTGACGCTCACCCCATAAAGACACTCACCCTA TAGAGGTAGTTGTCACACACACACACACACACACACACACACTGTTCACTTCCTAGCTCCTTTCCCCTCC ACCTTTCTTTCAGAGTTTGTTCTCATTTTTCAGAATTCTTCTGCTATCCTTCTCAAGTGGCCCTGAATGC CCTTTGTTAGCTCAATTTGGTGATGAGTTATCCCTGAACCTCTCCCAGTGTGCAGGAAGAAAAACTAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 89111. Forward Primer - name:089111_F_cDNA_D130072O21Rik, sequence:TCAAACAACCGCTGACTACATC; Reverse Primer - name:089111_N_SP6_cDNA_D130072O21Rik, sequence:CTAGTTTTTCTTCCTGCACACTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19091 same embryo
	EMAGE:19089 same embryo
	EurExpress:euxassay_015951 same experiment
	MGI:4828852 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source