EMAGE mouse gene expression spatial database

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EMAGE:19126

Mir200b microRNA 200b ( MGI:2676875)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019402. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019402_01	euxassay_019402_02	euxassay_019402_03	euxassay_019402_04

euxassay_019402_05	euxassay_019402_06	euxassay_019402_07	euxassay_019402_08	euxassay_019402_09

euxassay_019402_10	euxassay_019402_11	euxassay_019402_12	euxassay_019402_13	euxassay_019402_14

euxassay_019402_15	euxassay_019402_16	euxassay_019402_17	euxassay_019402_18	euxassay_019402_19

euxassay_019402_20	euxassay_019402_21	euxassay_019402_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19126_wholemount_strong.wlz

19126_wholemount_moderate.wlz

19126_wholemount_weak.wlz

19126_wholemount_possible.wlz

19126_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19126_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pituitary gland

weak

regional

weak expression: see section 12 13 14 15

vibrissa

weak

regional

weak expression: see section 03 04 05 19 20 21

ear

weak

regional

weak expression: see section 20 22

inner ear

weak

regional

weak expression: see section 02 05 16 17 18 19

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16 17

vomeronasal organ

strong

regional

strong expression: see section 11 13 14

stomach

weak

regional

weak expression: see section 08 09 10

midgut

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70320
Entity Detected:	Mir200b, microRNA 200b ( MGI:2676875)
Sequence:	sense strand is shown >T70320 TAATACTGCCTGGTAATGATGA
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-200b was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19125 same embryo
	EMAGE:19124 same embryo
	EMAGE:19127 same embryo
	EMAGE:19123 same embryo
	EurExpress:euxassay_019402 same experiment
	MGI:4826241 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source