EMAGE mouse gene expression spatial database

EMAGE:19129

Mir200c microRNA 200c ( MGI:3618750)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019403. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019403_01	euxassay_019403_02	euxassay_019403_03	euxassay_019403_04

euxassay_019403_05	euxassay_019403_06	euxassay_019403_07	euxassay_019403_08	euxassay_019403_09

euxassay_019403_10	euxassay_019403_11	euxassay_019403_12	euxassay_019403_13	euxassay_019403_14

euxassay_019403_15	euxassay_019403_16	euxassay_019403_17	euxassay_019403_18	euxassay_019403_19

euxassay_019403_20	euxassay_019403_21	euxassay_019403_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 05 06 07 08 09 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70321
Entity Detected:	Mir200c, microRNA 200c ( MGI:3618750)
Sequence:	sense strand is shown >T70321 TAATACTGCCGGGTAATGATGGA
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-200c was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19130 same embryo
	EMAGE:19131 same embryo
	EMAGE:19128 same embryo
	EMAGE:19132 same embryo
	EurExpress:euxassay_019403 same experiment
	MGI:4826242 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source