Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19153

Mir29c microRNA 29c ( MGI:2676906)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19153
euxassay_019368_01

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19153Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19153_wholemount_strong.wlz
19153_wholemount_moderate.wlz
19153_wholemount_weak.wlz
19153_wholemount_possible.wlz
19153_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19153_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
rib
moderate moderate
regionalmoderate expression: see section 01
meckel's cartilage
moderate moderate
regionalmoderate expression: see section 01
axial skeleton
moderate moderate
regionalmoderate expression: see section 01
basioccipital bone
moderate moderate
regionalmoderate expression: see section 01
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 01
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70381
Entity Detected:Mir29c, microRNA 29c ( MGI:2676906)
Sequence:sense strand is shown

>T70381
TAGCACCATTTGAAATCGGTTA
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-29c was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19152 same embryo
 EMAGE:19147 same embryo
 EMAGE:19148 same embryo
 EMAGE:19150 same embryo
 EMAGE:19151 same embryo
 EMAGE:19149 same embryo
 EurExpress:euxassay_019368 same experiment
 MGI:4826265 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS