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EMAGE:19175

Psma5 proteasome (prosome, macropain) subunit, alpha type 5 ( MGI:1347009)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19175 EMAGE:19175 EMAGE:19175 EMAGE:19175 EMAGE:19175
"Pseudo-wholemount" of euxassay_009266. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009266_01 euxassay_009266_02 euxassay_009266_03 euxassay_009266_04
EMAGE:19175 EMAGE:19175 EMAGE:19175 EMAGE:19175 EMAGE:19175
euxassay_009266_05 euxassay_009266_06 euxassay_009266_07 euxassay_009266_08 euxassay_009266_09
EMAGE:19175 EMAGE:19175 EMAGE:19175 EMAGE:19175 EMAGE:19175
euxassay_009266_10 euxassay_009266_11 euxassay_009266_12 euxassay_009266_13 euxassay_009266_14
EMAGE:19175 EMAGE:19175 EMAGE:19175 EMAGE:19175 EMAGE:19175
euxassay_009266_15 euxassay_009266_16 euxassay_009266_17 euxassay_009266_18 euxassay_009266_19
EMAGE:19175
euxassay_009266_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19175Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19175_wholemount_strong.wlz
19175_wholemount_moderate.wlz
19175_wholemount_weak.wlz
19175_wholemount_possible.wlz
19175_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19175_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T577
Entity Detected:Psma5, proteasome (prosome, macropain) subunit, alpha type 5 ( MGI:1347009)
Sequence:sense strand is shown

>T577
TCGAGCCTGTTGGCCTACTGGGGTGCTGTGGCGGTGTGGTTGATTAGGGTGCTGCTTTCAATCGTGTGGC
CTTTGGGAACCCCGCGTAGCCACTGCCGCCTCCTTCTGTCCTCGCCATGTTCCTCACTCGGTCCGAGTAC
GACAGGGGTGTGAATACTTTTTCTCCTGAAGGAAGATTATTTCAAGTGGAATATGCCATTGAGGCTATCA
AGCTTGGTTCTACAGCCATTGGCATCCAGACCTCAGAGGGTGTATGTCTAGCTGTGGAGAAGAGAATTAC
CTCCCCACTAATGGAGCCTAGCAGCATTGAGAAGATTGTAGAGATCGATGCTCATATAGGTTGTGCCATG
AGTGGGCTAATTGCTGATGCTAAAACTTTAATTGATAGAGCCAGAGTGGAGACACAGAACCACTGGTTCA
CCTATAATGAGACAATGACAGTTGAGAGTGTGACCCAGGCTGTGTCCAATCTGGCTCTGCAGTTTGGAGA
AGAAGATGCAGATCCAGGTGCTATGTCTCGTCCCTTTGGAGTAGCATTGTTGTTTGGAGGAGTTGATGAG
AAAGGACCCC
Notes:The probe template was PCR amplified from IMAGE:1885207 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1885207 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19173 same embryo
 EMAGE:19177 same embryo
 EMAGE:19176 same embryo
 EMAGE:19174 same embryo
 EMAGE:19172 same embryo
 EurExpress:euxassay_009266 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS