EMAGE mouse gene expression spatial database

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EMAGE:19178

Gpr107 G protein-coupled receptor 107 ( MGI:2139054)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009187. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009187_01	euxassay_009187_02	euxassay_009187_03	euxassay_009187_04

euxassay_009187_05	euxassay_009187_06	euxassay_009187_07	euxassay_009187_08	euxassay_009187_09

euxassay_009187_10	euxassay_009187_11	euxassay_009187_12	euxassay_009187_13	euxassay_009187_14

euxassay_009187_15	euxassay_009187_16	euxassay_009187_17	euxassay_009187_18	euxassay_009187_19

euxassay_009187_20	euxassay_009187_21	euxassay_009187_22	euxassay_009187_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19178_wholemount_strong.wlz

19178_wholemount_moderate.wlz

19178_wholemount_weak.wlz

19178_wholemount_possible.wlz

19178_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19178_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1195
Entity Detected:	Gpr107, G protein-coupled receptor 107 ( MGI:2139054)
Sequence:	sense strand is shown >T1195 TCCTCNAGNCTGTTGGCCTACTGGGATGCTGGAACAAACATGGCCGTCCCGGTACCCCTCGGCCGCTTCG GCTCCTTCTGCCTGCGGCTGCTACGACTGCTGGCGTTGCTGGAGCTGCTGGTCCACCCCGTCTTGGGCCG CGTCCATCACCTGGCGCTCAAAGATGATGTGCGGCATAAAGTCCATCTCAACACCTTCGGGTTCTTCAAG GATGGGTACATGGTGGTCAACGTCAGCAGCCTCTCTGTGAACGAGCCTGAGGGAGCCACGGACAAGGATG CTGAGATCGGATTCAGTCTGGATCGGACTAAGAACGATGGCTTTTCTTCTTACCTGGATGAAGATGTGAA TTACTGTATTTTAAAAAAGAAGTCCATGTCTTCTGTCACTCTTGTCATCTTAGACATCTCTGGAAGCATA GTCAAGGTCAGATCCCCACCAGAAGCTGGTAAGCAGTTACCAGAGATTGTCTTCAGCAAGGATGAGAAAA TCCTGAGTCAGAGCCAGGAGCCTGCTGTCAGCTCCAACCCCAAAGACAGTGAGGCT
Notes:	The probe template was PCR amplified from IMAGE:2182273 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2182273 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19181 same embryo
	EMAGE:19183 same embryo
	EMAGE:19182 same embryo
	EMAGE:19180 same embryo
	EMAGE:19179 same embryo
	EurExpress:euxassay_009187 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source