EMAGE mouse gene expression spatial database

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EMAGE:19179

Ctbs chitobiase, di-N-acetyl- ( MGI:1921495)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009189. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009189_01	euxassay_009189_02	euxassay_009189_03	euxassay_009189_04

euxassay_009189_05	euxassay_009189_06	euxassay_009189_07	euxassay_009189_08	euxassay_009189_09

euxassay_009189_10	euxassay_009189_11	euxassay_009189_12	euxassay_009189_13	euxassay_009189_14

euxassay_009189_15	euxassay_009189_16	euxassay_009189_17	euxassay_009189_18	euxassay_009189_19

euxassay_009189_20	euxassay_009189_21	euxassay_009189_22	euxassay_009189_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19179_wholemount_strong.wlz

19179_wholemount_moderate.wlz

19179_wholemount_weak.wlz

19179_wholemount_possible.wlz

19179_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19179_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 10 11 13 moderate expression: see section 12 14

telencephalon ventricular layer

strong

regional

strong expression: see section 10 11 moderate expression: see section 02 03 04 05 06 07 08 09 12 14 15 16 17 18 19 20 21 22

medulla oblongata alar plate ventricular layer

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 02 03 04 05 06 10 11 12 13 14 15 16 17 18

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 02 03 04 05 06 10 11 12 13 14 15 16 17 18

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 02 03 04 05 06 10 11 12 13 14 15 16 17 18

pons ventricular layer

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 02 03 04 05 06 10 11 12 13 14 15 16 17 18

midbrain ventricular layer

strong

regional

strong expression: see section 10 11 13 moderate expression: see section 06 07 08 09 12 14 15 16

spinal cord ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1207
Entity Detected:	Ctbs, chitobiase, di-N-acetyl- ( MGI:1921495)
Sequence:	sense strand is shown >T1207 TCGAGNCTGTTGGCCTACTGGAGGCATGGCGCTGTGTGGCCTCCCGGAATTCACTCTGCTGCTGCTGCCG CTGTTGGCACGGCTCTCGGCCGGGGACTGCCCGTGCTCCGAGGCTGCTCTCTGCCAACCGATCCGCCACC GTCCCGACTTCGAGGTCTTTGTATTTGATGTTGGACAGAAAACCTGGAAATCTTATGACTGGTCACAGAT TACAACTGTGGCAGCTTTTGGAAAATATGACCCAGAACTTATGTGCTATGCTCATTCAAAAGGAGCCAGG GTAGTGCTAAAAGGTGACATCTCTCTGAAGAATATCATTGATCCTACCTTCAGAGCATCGTGGATAGCTC AGAAAGTTGATTTAGCCAAAGCACAGTATATGGATGGGATTAATATAGACATAGAGCAAGAAGTTAACTG CTCATCACCTGAATATGAAGCACTGACAGCTCTGGTCAAAGAAACCACAGAGAGTTTCCAACGTGAGATC GAGGGATCACAGGTAACCTTTGATGTAGCTTGGTCACCAAAGCGCATAGACAAAAGGTGCTATAATTACA CTGGGATCGCAGAT
Notes:	The probe template was PCR amplified from IMAGE:2182852 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2182852 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19181 same embryo
	EMAGE:19183 same embryo
	EMAGE:19182 same embryo
	EMAGE:19180 same embryo
	EMAGE:19178 same embryo
	EurExpress:euxassay_009189 same experiment
	MGI:4824098 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source