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EMAGE:19180

Zfp954 zinc finger protein 954 ( MGI:1917764)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19180 EMAGE:19180 EMAGE:19180 EMAGE:19180 EMAGE:19180
"Pseudo-wholemount" of euxassay_009185. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009185_01 euxassay_009185_02 euxassay_009185_03 euxassay_009185_04
EMAGE:19180 EMAGE:19180 EMAGE:19180 EMAGE:19180 EMAGE:19180
euxassay_009185_05 euxassay_009185_06 euxassay_009185_07 euxassay_009185_08 euxassay_009185_09
EMAGE:19180 EMAGE:19180 EMAGE:19180 EMAGE:19180 EMAGE:19180
euxassay_009185_10 euxassay_009185_11 euxassay_009185_12 euxassay_009185_13 euxassay_009185_14
EMAGE:19180 EMAGE:19180 EMAGE:19180 EMAGE:19180 EMAGE:19180
euxassay_009185_15 euxassay_009185_16 euxassay_009185_17 euxassay_009185_18 euxassay_009185_19
EMAGE:19180 EMAGE:19180 EMAGE:19180 EMAGE:19180
euxassay_009185_20 euxassay_009185_21 euxassay_009185_22 euxassay_009185_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19180Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19180_wholemount_strong.wlz
19180_wholemount_moderate.wlz
19180_wholemount_weak.wlz
19180_wholemount_possible.wlz
19180_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19180_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 moderate expression: see section 01
facial vii ganglion
strong strong
regionalstrong expression: see section 02 03 16 17 18
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 04 moderate expression: see section 15
trigeminal v ganglion
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 15 16 17 18 19 20
vagus x ganglion
strong strong
regionalstrong expression: see section 04 moderate expression: see section 13 14 15
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 04 14 15
trigeminal v nerve
strong strong
regionalstrong expression: see section 07 moderate expression: see section 14
spinal cord
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 05 11
cervical ganglion
strong strong
regionalstrong expression: see section 04 05 12 13
thoracic ganglion
strong strong
regionalstrong expression: see section 10 11 moderate expression: see section 07 09
dorsal root ganglion
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 moderate expression: see section 15
retina
strong strong
regionalstrong expression: see section 01 02 21 22 23
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 07 08 09 10 11 13 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1204
Entity Detected:Zfp954, zinc finger protein 954 ( MGI:1917764)
Sequence:sense strand is shown

>T1204
GCTTGGCCTACTGGGNTAACTTCCTGGCACCCGTCAGTATGGCTCACAGAAAACTGGTGTCATCTGCTTT
GGCCATGGCAGGAAAGCCGGCACGTTAAAAGCGTTTGTCCCCCGTCACAGAGACGGATCTGAGGTTTGCG
ACAGCTCGTGTGATCTGATCCAGGCTTCGAGTGCGGATGGCGACTTCCCAGGGCCCCACCATCCTCTGGA
GCCCGATGGTGATTGTGGAGGCTGCGGGTGGAGCCCTGCCCCAGGTTTCCCAGTATTCAGTGAAACCTGC
CAACCAGGAAGAGGGCTGCGTGTCCTTTGAGGATGTGGCTGTGTACTTCTCCTGGGAGGAATGGACACTG
CTCGATGACTCTCAAAGGCTCCTGTACCAGACTGTGATGACGGAGATCTTCACACTGGTGTCTTCACTGA
GACTTGAACTTTCTGGGATTCATGATTCAACTCAGGNGGAATCTTGTGGAGATCTCTCTGTGCCTGCACT
AAGATTCCTGACTCCGTGTTTATGAGACAGGTTGTTGGATCAAAGTGCGAG
Notes:The probe template was PCR amplified from IMAGE:2182489 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2182489 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19181 same embryo
 EMAGE:19183 same embryo
 EMAGE:19182 same embryo
 EMAGE:19178 same embryo
 EMAGE:19179 same embryo
 EurExpress:euxassay_009185 same experiment
 MGI:4822763 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS