EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19225

Panx1 pannexin 1 ( MGI:1860055)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009252. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009252_01	euxassay_009252_02	euxassay_009252_03	euxassay_009252_04

euxassay_009252_05	euxassay_009252_06	euxassay_009252_07	euxassay_009252_08	euxassay_009252_09

euxassay_009252_10	euxassay_009252_11	euxassay_009252_12	euxassay_009252_13	euxassay_009252_14

euxassay_009252_15	euxassay_009252_16	euxassay_009252_17	euxassay_009252_18	euxassay_009252_19

euxassay_009252_20	euxassay_009252_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19225_wholemount_strong.wlz

19225_wholemount_moderate.wlz

19225_wholemount_weak.wlz

19225_wholemount_possible.wlz

19225_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19225_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 07 08 09 15 16

brain

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

weak

regional

weak expression: see section 05 06

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07 16

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 15 16 17 18 19 20

vagus x ganglion

weak

regional

weak expression: see section 15

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 16

trigeminal v nerve

weak

regional

weak expression: see section 09 15

spinal cord

weak

regional

weak expression: see section 08 09 10 11 12 13 14

neural retina

weak

regional

weak expression: see section 02 03 04 20 21

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2404
Entity Detected:	Panx1, pannexin 1 ( MGI:1860055)
Sequence:	sense strand is shown >T2404 TGGCCTCGAGCCAGATTCGGCACGAGGGTTTCCTTCTTGAATTTCAGCCTGTGACTTCTGTGGCTGACGC ACCCCTGTTGGATCTGTAGTTAGTTTGCAGTAGATGTTTTTGGTATTGATGTAGTGTGTGTCCTACCAAT CTCTAATAGACATAATGGCCAACAGCGTCATAGAAGAATAGATTAGAAACGTCCCACAAGAGTAAGTGTG CTTTGTGTGAAGTCCTCATTGCAGGGCTGTTAAGAGCACAGAGCCTCAGCCACAGAGGAGCAGCATTCTG GACTGGCAGGTGCTAGAGGACCTGGGGCTTCTAGAGAGCAAGCACATGGAAGATGCTTTGTTTTGTGAGG TAATAAACATGTGAGGATGAAACTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1225336 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1225336 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19223 same embryo
	EMAGE:19222 same embryo
	EMAGE:19224 same embryo
	EurExpress:euxassay_009252 same experiment
	MGI:4827038 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source