EMAGE mouse gene expression spatial database

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EMAGE:19240

Ppp2r3c protein phosphatase 2, regulatory subunit B'', gamma ( MGI:1930009)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002065. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002065_01	euxassay_002065_02	euxassay_002065_03	euxassay_002065_04

euxassay_002065_05	euxassay_002065_06	euxassay_002065_07	euxassay_002065_08	euxassay_002065_09

euxassay_002065_10	euxassay_002065_11	euxassay_002065_14	euxassay_002065_15	euxassay_002065_16

euxassay_002065_17	euxassay_002065_18	euxassay_002065_19	euxassay_002065_20	euxassay_002065_21

euxassay_002065_22	euxassay_002065_23	euxassay_002065_24	euxassay_002065_25	euxassay_002065_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19240_wholemount_strong.wlz

19240_wholemount_moderate.wlz

19240_wholemount_weak.wlz

19240_wholemount_possible.wlz

19240_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19240_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5478
Entity Detected:	Ppp2r3c, protein phosphatase 2, regulatory subunit B'', gamma ( MGI:1930009)
Sequence:	sense strand is shown >T5478 GGCTTCTTCTGGCCTTTAGAAATCCGGCTTGGTGAGCTGCGACACCTCCCCAAGGCTTCCGCCCGTGCCC AAGATGGCGTCCAGGCGTCCTAATTAACTTCCACCTGTCTTGGCGAAGTCTGACGTCGCGGCTGGCAGTG GGTGGAGCTCTTAACAAACTGCAAATGTAGGGTCCTCTGAGCGGGAGTCAACCATGGACTGGAAAGACGT GCTTCGCCGGCGGTTAGCGTCGCCCAACACGGATCCAAAGAGGAAAAAAAGCGAACAAGAATTAAAAGAT GAAGAAATGGATTTATTTACCAAATACTACTCAGAGTGGAAAGGAGGTAGAAAAAACACAAACGAGTTCT ATAAGACCATACCCCGGTTTTATTACAGGTTGCCAGCTGAAGATGAAGTCTTACTACAGAAATTACGAGA GGAATCTAGAGCTGTCTTTCTACAGAGGAAAAGCAGAGAACTCTTAGATAACGAAGAGTTGCAGAACTTA TGGTTTTTGCTGGATAAACACCAGATACCACCTATGATTGGAGAGGAAGCAATGATCAATTATGAAAATT TTTTGAAGGTTGGTGAAAAGGCTGGACCAAAGTGCAAGCAATTTTTTACTGCAAAAGTCTTTGCCAAACT CCTTCATACAGATTCATATGGACGAATTTCCATCATGCAGTTCTTTAACTACGTCATGCGAAAAGTTTGG CTGCATCAGACAAGAATAGGACTCAGTTTATATGATGTTGCTGGCCAAGGATACCTTCGGGAATCAGACC TGGAGAACTACATCCTGGAGCTGATCCCGACGCTGCCACAGCTGGACGGGCTGGAGAAGTCCTTCTACTC CTTCTATGTCTGCACTGCAGTCAGGAAGTTCTTCTTCTTCTTGGACCCTCTAAGAACAGGGAAGATCAAA ATTCAAGATATTTTGGCATGCAGTTTTCTAGATGATTTACTGGAGCTAAGAGATGAGGAATTGTCCAAAG AAAGTCAAGAAACAAATTGGTTTTCTGCTCCTTCTGCCCTGAGGGTCTATGGTCAGTATTTGAATCTTGA TAAAGATCATAATGGCATGCTAAGTAAAGAGGAGCTCTCCCGTTACGGAACAGCAACCATGACCAATGTC TTCTTAGACCGAGTTTTCCAGGAGTGTCTCACTTACGATGGAGAAATGGACTATAAGACCTACCTGGACT TTGTTCTTGCCTTAGAAAACAGAAAGGAGCCTGCAGCTCTGCAGTACATTTTCAAACTGCTGGACATTGA GAACAAGGGATACCTGAATGTCTTTTCCCTTAATTATTTCTTTAGGGCCATACAAGAACTAATGAAAATC CATGGACAGGATCCTGTGTCATTTCAAGATGTCAAGGATGAAATCTTTGACATGGTCAAACCAAAGGATC CTTTGAAAATCTCACTTCAGGATTTAATCAACAGTAATCAAGGAGACACAGTCACTACCATTCTAATTGA TCTCAATGGCTTCTGGACCTACGAGAACAGAGAAGCCCTTGTTGCAAATGACAACGAGAACTCTGCAGAT CTTGATGACACATGATCTCTGCAAAATAGACTTCTTCATAAAGATGCTTGAATGCTGCATGCAACACTGT TGAAGCAGAATCCTTAGAAACGTTCTAAATAAAACTCATCACATGCCTGTACAACACAGCAAATATCTTG CTTACTAGAGTGTTGGTTGCAGTCAGAACAATTTGAAAGTAATAGGACCAACTATTGGTTTTTTATTAAG TGGTAAAGTAAGTCCTAGTTCTGTCTAACTCAGAAATGGAAAGTAAGATTTGCTGTAGTTAGCCGTGGAG TTTATTCATGTGGAATTACAATATTTGTTCTTCAAGAAGATTGGGGACAGGAGACTTCCCATCAGAGTAG CACCATTGCATTTCTGATTCAAAGCTTTAGGTGTGGCTTTGGTTGTTTTGAGACATGGTCTCACTATGTA GCCCTGACTGGCCTGGAACTTGCTATGTGGACCAAGTTGGCCTTTAACCTTGAACTCTTAAGAGAGACAC CTGCCTCTGCCTCCCAGGTGCTGAGGTTAAAGGTGAGTGCCTCTGTGCCTATCGTTTTTGGGGTTTTTGT TGTTGTTTTTGTATTTCTTGTGTGTGCACACCAGAGCACACATGGAGGTCAGTAGACATTGTTGTGATTC TTGTCTTTCCCCACGTGGGTTCTGGAGATCAGATTCAGGTTCTCGGCTTCCCAGCAAGCGCTTGTACCCA GCATCCTCTTGCCTTCCCAAGATTTTTTTGTTTTGTTTTTTTTGTTTTATTAAACTCATCAGATTGAATG AGATTTTGTCTAGGTCTATTAAGCATTTACAAAACTAACTTCTGTTAAAGGTTTTGCTAGAGAATTTACT AATTCAGTATACTTATGTGTTTATGGTAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5375405 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5375405 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19242 same embryo
	EMAGE:19243 same embryo
	EMAGE:19241 same embryo
	EurExpress:euxassay_002065 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source