EMAGE mouse gene expression spatial database

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EMAGE:19242

Gstm4 glutathione S-transferase, mu 4 ( MGI:95862)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002073. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002073_01	euxassay_002073_02	euxassay_002073_03	euxassay_002073_04

euxassay_002073_05	euxassay_002073_06	euxassay_002073_07	euxassay_002073_08	euxassay_002073_09

euxassay_002073_10	euxassay_002073_11	euxassay_002073_12	euxassay_002073_13	euxassay_002073_14

euxassay_002073_15	euxassay_002073_16	euxassay_002073_17	euxassay_002073_18	euxassay_002073_19

euxassay_002073_20	euxassay_002073_21	euxassay_002073_22	euxassay_002073_23	euxassay_002073_24

euxassay_002073_25	euxassay_002073_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19242_wholemount_strong.wlz

19242_wholemount_moderate.wlz

19242_wholemount_weak.wlz

19242_wholemount_possible.wlz

19242_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19242_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18 19 20

testis

strong

regional

strong expression: see section 05 06 07 08 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5465
Entity Detected:	Gstm4, glutathione S-transferase, mu 4 ( MGI:95862)
Sequence:	sense strand is shown >T5465 CCACGCGTCCGGTTCCTGGGCGGGCAGGATCACGCCGGGCACCAGCCCCGCCCAGGGACCCGAGGGCACT AGGATAAAGTCAAAGTTGCTCACACCGGAGGCCGGTGGACTCAGTCTCAGGCACCAGCATCATGCCTATG ACACTGGGTTACTGGGACATCCGTGGGCTAGCTCACGCTATTCGGCTGCTCCTAGAATACACAGGCTCAA GCTATGAAGAGAAGAGATACACCATGGGAGACGCTCCTGACTATGACCGAAGCCAGTGGCTGAGTGAGAA GTTCAAATTGGGCCTGGACTTTCCCAATTTGCCTTACTTGATTGATGGGTCACACAAGATCACGCAGAGC AATGCCATCCTGCGCTACATTGCCCGCAAGCACAACCTGTGTGGGGAGACAGAGGAAGAGAAGATTCGCG TGGACATTTTGGAGAACCAGGCTATGGATGTCTCCAATCAGCTGGCTCGAGTCTGTTACAGCCCAGACTT TGAGAAACTGAAGGTGGAATACTTGGAGCAGCTCCCTGGAATGGTGAAGCTCTTCTCACAGTTCCTGGGC CAGCGGACATGGTTTGTTGGTGAAAAGATTACTTTTGTAGATTTCCTGGCTTACGATATCCTGGACCTGC ACCTTATATTCGAACCCACGTGCCTGGACGCCTTCCCAAACCTGAAGGACTTTGTGGCCCGCTTTGAGGT ACTGAAGAGGATCTCTGCTTACATGAAGACCAGCCGCTTCCTCCGAACACCCCTATATACAAAGGTGGCC ACTTGGGGCAATAAGTAGAGCCTTGACTGGGCAGGAAGTGGGAACCGGGGGTTCTGGGAACAGTTGAACT TCCTGTAGCCCTAGTGCTGCTTTCTGTCCATCCCCTTCCTGACCCCAGAGTGTCAAAGGTCCTCTTTTCT TTCATCCAGTCCCTGCCCTTTCAAACCCTCTAAAGCCTAGGCTCCTAGTTTTCCTTTAGCAAAATGCCCT TCTAGCATGACTGTGTCCCGCGCCCACTTGATGGTCTTGCCTGCCAGCAGCTGTGCTGTTGTGAAGAGTT GGGACTCTCCATCAGCACTCAGCCTGGGGCTCCCCATGCTTGTCTGGAGACCAGAGACGGCTGGTGTGTG AATTTGGTCTCTGCACAGCTCTTTTGGGTCCCTCCTGTAAAGCCTGAACCACACTGGCTCTGGCTGCCGC ACTACCAGCTTTTACTACTATCTCCAGTGGCTGCCTAGTGACCCGGGCAGAGGCTGAGTTCACAGGGATT TTAGTTGGATAGGCAGGGGTTTGGCCCTTCCTAGCCCCACCTGTTTGTTTCTCAGGAGGCAGCTGCAGAA GGCTCTGTGGAGCTCAAAGGGAGCTTAGATCTCCTTTATGCTAGCAGCACTGAGATTTGTCATGCAGGTC TCAGTGGTGAGGATCCAGGCTGATAGGAGTCCCCAGCAGAAAGCCAGGATCCTCTCTGCCCACTGTGCTA TGGCTGCCTTATATCTATATGTCTCCAGGATCCTGTCTCTGATGTCTTCAGAGTATCCCGTCTTGGTCAC CAGGGATGGGGGCCATCTTGGTTAATCCCTCCTCTTTGTGAGCCCCCGTGAAATAAATTTCTTCATGCTT TCGTAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5373062 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5373062 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19240 same embryo
	EMAGE:19243 same embryo
	EMAGE:19241 same embryo
	EurExpress:euxassay_002073 same experiment
	MGI:4825262 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source