EMAGE mouse gene expression spatial database

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EMAGE:19254

Tbpl1 TATA box binding protein-like 1 ( MGI:1339946)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019419. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019419_01	euxassay_019419_02	euxassay_019419_03	euxassay_019419_04

euxassay_019419_05	euxassay_019419_06	euxassay_019419_07	euxassay_019419_08	euxassay_019419_09

euxassay_019419_10	euxassay_019419_11	euxassay_019419_12	euxassay_019419_13	euxassay_019419_14

euxassay_019419_15	euxassay_019419_16	euxassay_019419_17	euxassay_019419_18	euxassay_019419_19

euxassay_019419_20	euxassay_019419_21	euxassay_019419_22	euxassay_019419_23	euxassay_019419_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19254_wholemount_strong.wlz

19254_wholemount_moderate.wlz

19254_wholemount_weak.wlz

19254_wholemount_possible.wlz

19254_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19254_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 09 17 18 19 20 21 22 23 24

facial vii ganglion

moderate

regional

moderate expression: see section 03 04 19 20 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21

vagus x ganglion

moderate

regional

moderate expression: see section 07 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 08 14

cervical ganglion

moderate

regional

moderate expression: see section 08 15

thoracic ganglion

weak

regional

weak expression: see section 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 05 06 07 08 09 10 14 15 weak expression: see section 16

neural retina

weak

regional

weak expression: see section 02 03 04 23 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2546
Entity Detected:	Tbpl1, TATA box binding protein-like 1 ( MGI:1339946)
Sequence:	sense strand is shown >T2546 TGGCCTCGAGCCAGATTCGGCACGAGGCCGAGCTCGAGCCTTGGGGGGCTCCTAGCAACGGGCCTGGGCG GGAGTTCCATGGAGACTGGGGAGCGGACCCGTTTTATCTTCATCCTTGTCCTCCAGCTTCTTCTCCGCGT CCGACGCAACCAGCAGCAGCGCTGCCGCCGTGTCCTTTACGACCGCCCCGTCTTCCCACGGATGTGATCT TCGTGGTGGGAACCAAGTTTCTAAACTACCCCAATGGATGCAGACAGTGATGTTGCATTGGACATTTTAA TTACAAATGTAGTCTGTGTTTTTAGAACAAGATGCCATTTGAACTTAAGGAAGATTGCTTTGGAGGGAGC AAATGTAATTTATAAGCGTGATGTTGGGAAAGTATTAATGAAGCTTAGAAAACCTAGAATTACAGCTACA ATTTGGTCCTCAGGAAAAATTATTTGCACTGGAGCAACAAGTGAAGAAGAAGCTAAATTTGGTGCCAGAC GTTTAGCCCGTAGTCTGCAGAAACTAGGTTTTCAGGTCATCTTCACAGATTTTA
Notes:	The probe template was PCR amplified from IMAGE:1382180 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1382180 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19258 same embryo
	EMAGE:19256 same embryo
	EMAGE:19253 same embryo
	EMAGE:19257 same embryo
	EMAGE:19255 same embryo
	EurExpress:euxassay_019419 same experiment
	MGI:4828616 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source