EMAGE mouse gene expression spatial database

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EMAGE:19265

Spata5 spermatogenesis associated 5 ( MGI:1927170)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009237. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009237_01	euxassay_009237_02	euxassay_009237_03	euxassay_009237_04

euxassay_009237_05	euxassay_009237_06	euxassay_009237_07	euxassay_009237_08	euxassay_009237_09

euxassay_009237_10	euxassay_009237_11	euxassay_009237_12	euxassay_009237_13	euxassay_009237_14

euxassay_009237_15	euxassay_009237_16	euxassay_009237_17	euxassay_009237_18	euxassay_009237_19

euxassay_009237_20	euxassay_009237_21	euxassay_009237_22	euxassay_009237_23	euxassay_009237_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19265_wholemount_strong.wlz

19265_wholemount_moderate.wlz

19265_wholemount_weak.wlz

19265_wholemount_possible.wlz

19265_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19265_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T568
Entity Detected:	Spata5, spermatogenesis associated 5 ( MGI:1927170)
Sequence:	sense strand is shown >T568 CCTCGAGNCTGTTGGCCTACTGGAATTATAAGCCAATTCTACGGTGAGACTGAAGCAAGGTTACGCCAGA TATTTGCTGAAGCTACTCTACGACACCCATCAATTATTTTTATTGATGAACTGGATGCACTTTGCCCTAA AAGAGAAGGAGCTCAGAGTGAAGTGGAAAAGAGAGTTGTAGCTTCACTCTTAACATTGATGGATGGCATT GGCTCAGAAGGAAGTGAAGGACGGGTATTAGTCCTTGGGGCCACAAATCGCCCTCAAGCACTAGATGCTG CACTCAGACGACCTGGGAGATTTGATAAAGAGATTGAGATTGGCATTCCTAATGCCCAAGACCGGCTTGA TATTCTCCAGAAACTACTCCGAAGGGTGCCACATTTGCTCACTAAAGCTGAGCTTCTGAGGTTGGCAAAT AATGCCCATGGTTATGTTGGCGCAGACTTGAAAGCGTTGTGTAATGAGGCAGGTCTGCATGCCTTGCGAA GAGTCCTGAGGAAACAGCCTAACCTCCCTGATAGCAAGGTGGCTGGCATGGTGAAGATTACTCTAAATGA TTTCTTACAGGG
Notes:	The probe template was PCR amplified from IMAGE:1499779 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1499779 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19262 same embryo
	EMAGE:19266 same embryo
	EMAGE:19264 same embryo
	EMAGE:19263 same embryo
	EurExpress:euxassay_009237 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source