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EMAGE:19277

Rps3a ribosomal protein S3A ( MGI:1202063)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:19277 EMAGE:19277 EMAGE:19277 EMAGE:19277 EMAGE:19277
"Pseudo-wholemount" of euxassay_009203. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009203_01 euxassay_009203_02 euxassay_009203_03 euxassay_009203_04
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euxassay_009203_05 euxassay_009203_06 euxassay_009203_07 euxassay_009203_08 euxassay_009203_09
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euxassay_009203_10 euxassay_009203_11 euxassay_009203_12 euxassay_009203_13 euxassay_009203_14
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euxassay_009203_15 euxassay_009203_16 euxassay_009203_17 euxassay_009203_18 euxassay_009203_19
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euxassay_009203_20 euxassay_009203_21 euxassay_009203_22 euxassay_009203_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:19277Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
19277_wholemount_strong.wlz
19277_wholemount_moderate.wlz
19277_wholemount_weak.wlz
19277_wholemount_possible.wlz
19277_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:19277_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3463
Entity Detected:Rps3a, ribosomal protein S3A ( MGI:1202063)
Sequence:sense strand is shown

>T3463
TGGCCTCGAGCCAGATTCGGCACGAGGGTCGGCACCATGGCGGTCGGCAAGAACAAGCGCCTGACGAAAG
GTGGCAAGAAGGGAGCTAAGAAGAAAGTGGTCGATCCATTTTCTAAGAAAGACTGGTACGATGTGAAAGC
TCCAGCCATGTTCAATATTAGGAACATCGGGAAGACACTAGTCACGAGGACTCAAGGAACCAAAATTGCC
TCTGATGGCCTCAAGGGTCGTGTGTTTGAAGTGAGCCTTGCTGATCTACAGAATGATGAAGTTGCGTTTA
GAAAATTCAAGCTAATCACTGAGGACGTTCAGGGCAAAAACTGTCTGACTAACTTCCATGGTATGGATCT
TACCCGGGACAAAATGTGCTCCATGGTCAAGAAGTGGCAGACCATGATCGAAGCTCACGTTGATGTCAAG
ACGACCGATGGGTATTTGCTCCGACTTTTCTGTGTTGGCTTCACTAAGAAACGCAACAACCAGATAAGAA
AGACATCCTATGCGCAGCACCAGCAAGTCCGCCAGATCCGGAAGAAGATGATGGAAATCATGACCCGAGA
GGTGCAG
Notes:The probe template was PCR amplified from IMAGE:330191 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:330191 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:19278 same assay
 EMAGE:19276 same embryo
 EMAGE:19275 same embryo
 EMAGE:19274 same embryo
 EurExpress:euxassay_009203 same experiment
 EMAGE:19279 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS