EMAGE mouse gene expression spatial database

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EMAGE:19281

Mfsd10 major facilitator superfamily domain containing 10 ( MGI:1915544)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002092. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002092_01	euxassay_002092_02	euxassay_002092_03	euxassay_002092_04

euxassay_002092_05	euxassay_002092_06	euxassay_002092_07	euxassay_002092_08	euxassay_002092_09

euxassay_002092_10	euxassay_002092_11	euxassay_002092_12	euxassay_002092_13	euxassay_002092_14

euxassay_002092_15	euxassay_002092_16	euxassay_002092_17	euxassay_002092_18	euxassay_002092_19

euxassay_002092_20	euxassay_002092_21	euxassay_002092_22	euxassay_002092_23	euxassay_002092_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19281_wholemount_strong.wlz

19281_wholemount_moderate.wlz

19281_wholemount_weak.wlz

19281_wholemount_possible.wlz

19281_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19281_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 01

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5483
Entity Detected:	Mfsd10, major facilitator superfamily domain containing 10 ( MGI:1915544)
Sequence:	sense strand is shown >T5483 CACAGGTCCCAGCTGGGTAGGTGGGAAGCAAAGGTTTCGATATCGTGTGAGGAGGCCGAAGTGGTACACG AACAGGAGCTCAGGGCGCCTACGACTCCCCAACCAGGGATTCCCGTCTTCTCCCCGTTACCCGCTTCACA ACAGGGGGCGCCCTGACCACTGCGGCCCTGAGCTTACTGCTGCCCTAGGGTCGGAGCTTCCACCATGGGC TGGGCAGGGGATGCGGGCTGTACGCCTCGTCCGCCTATACGGCCTCGGCCCGCGTCCGAGCGTCGCGTGA TCATCGTGCTCTTCCTCGGCCTGCTACTCGACCTCCTGGCGTTCACGCTGTTGCTGCCTCTGCTGCCTGG GCTGCTGGAGAGACACGGCCGCGAACAAGACCCCCTCTATGGCTCCTGGCAGCGTGGGGTAGACTGGTTT GCTTCAGCCATCGGGATGCCAGCGGAAAAGAGGTACAACAGTGTCCTGTTCGGAGGTCTCATCGGCTCGG CCTTCTCCCTCCTGCAGTTCTTCTCCGCACCACTCACAGGAGCTGCCTCTGATTACCTGGGGAGGCGCCC AGTGATGATGTTGTCCCTGACAGGTTTGGCCATCTCCTATGCAGTGTGGGCTACCTCTCGGAGCTTTAAG GCCTTCTTGGCCTCCAGGGTGATCGGAGGCATCAGCAAGGGGAACGTTAACCTCTCCACAGCCATTGTCG CTGACCTGGGCTCACCTCCAACCCGCAGCCAAGGCATGGCAGTCATCGGGGTAGCCTTCTCCTTGGCCTT TACTCTGGGTCCCATGCTGGGAGCATTCCTGTCTGTGGAAATGGTGCCCTGGATCAGCCTACTTTTTGCC ATCTCCGACATGTTGTTCATCTTCTGTTTCTTGCCAGAGACACTGCCCCAGGAAAAGCGGGCATCTTCTG TCACCCTGGGCTTCCACACTGCTGCCCACCTGCTCAGCCCCCTGGCCCTGCTTCGCTTTGCGGCTGTCAC TCACAGTCAGGACCCGCCTGCTGAGCACAGACTCAGAAACTTACGCCGTTTGGGTCTTGTCTACTTCCTC TACCTCTTCCTGTTCTCAGGCCTGGAGTACACACTCAGCTTCCTGGCACATCAGCGCTTCCAGTTCAGCA GCCTGCAGCAGGGCAAGATGTTTTTCTTCATCGGCCTCACCATGGCCACCATACAGGGCACCTACGCCCG GCGGATCAGCCCTGGCAAGGAAGCTGCAGCTGTGACGAGGGCAATGCTGCTGCTAGTACCAGCCTTCCTC TTAATTGGCTGGGCACACTCGCTGCCTACGTTGGGCTTGGGACTGATGCTCTACTCCTTTGCTGCTGCTG TTGTGGTGCCTGGTCTGTCCACCATGGTCTCCAGTTATGGTTCTCCAGGGCAAAAAGGCACAATCATGGG CATACTGCGGAGCCTAGGCGCCCTAGGCAGGGCGTTGGGGCCGGTGGTGGCTGCCTCAGTGTACTGGCTG ACTGGGGCCCAGGTCTGCTTCACTGTGTGCTCGGCCCTCTTTCTGCTCCCCTTCCTACTCCTGTGGAAGC TGAAGCATCCAGCAGAGACTTCCAAGGAGGAGTAGCTGAGCTGCCACACCCAACCCCAGCTGTCTGGCTG CCAGGTGGGAACCTGGGCCCAGACCACTCAGCTCCTAGCACACTCCTCCCACCGGGCCCAGGGAGGCTCG CCACGCCCCTCCTCTCTCTGGATGGCAACCCAGCAGGAAGCCTGGGCAGCTACACCCTTTTCTTACTCCA AAACCTTTTAGGCTTCCAGAATTTTTTTTATATCTTATTCTTCTGAATGACCAAATAAAGCAGCTATTTT GTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5376230 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5376230 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19284 same embryo
	EMAGE:19285 same embryo
	EMAGE:19283 same embryo
	EMAGE:19282 same embryo
	EMAGE:19280 same embryo
	EurExpress:euxassay_002092 same experiment
	MGI:4826181 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source