EMAGE mouse gene expression spatial database

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EMAGE:19282

Gna14 guanine nucleotide binding protein, alpha 14 ( MGI:95769)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002103. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002103_01	euxassay_002103_02	euxassay_002103_03	euxassay_002103_04

euxassay_002103_05	euxassay_002103_06	euxassay_002103_07	euxassay_002103_08	euxassay_002103_09

euxassay_002103_10	euxassay_002103_11	euxassay_002103_12	euxassay_002103_13	euxassay_002103_14

euxassay_002103_15	euxassay_002103_16	euxassay_002103_17	euxassay_002103_18	euxassay_002103_19

euxassay_002103_20	euxassay_002103_21	euxassay_002103_22	euxassay_002103_23	euxassay_002103_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19282_wholemount_strong.wlz

19282_wholemount_moderate.wlz

19282_wholemount_weak.wlz

19282_wholemount_possible.wlz

19282_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19282_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

foregut-midgut junction

moderate

regional

moderate expression: see section 10 11 12 13

dorsal root ganglion

weak

regional

weak expression: see section 08 09 10 16 17

esophagus

moderate

regional

moderate expression: see section 12 13 weak expression: see section 11

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10

hindgut

moderate

regional

moderate expression: see section 11 12 13 14 15 16

rectum

moderate

regional

moderate expression: see section 12 13 14

midgut

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18

kidney calyx

weak

regional

weak expression: see section 08 09 10 17 18 19 20

lung

moderate

spotted

moderate expression: see section 05 06 07 08 09 15 16 17 18 19 20

trachea

strong

regional

strong expression: see section 12 13

axial skeleton

strong

regional

strong expression: see section 12 13 14 15 17

axial skeleton tail region

strong

regional

strong expression: see section 09 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5482
Entity Detected:	Gna14, guanine nucleotide binding protein, alpha 14 ( MGI:95769)
Sequence:	sense strand is shown >T5482 CTTCCATTCCGCAAAAGTCTACGGGTTGAGGTTTTCGGAACTTTGCAAACAGGGACTAGCTGGCCCTTTA TTTGCAAGCCAGGCCACACCTGCTGCTAGCCTGCGACACCCTACAGTCCCGGAGAGACCATTCTAGCAGC AGCACCGACGCCCCAACTTTTGATTCGCGGTGTGCGTTGCCTACCTGGGTACCCAGCTACAGTGTCCGGG GACGCACATCTTCTGGAGACAGCGCCAATCACACTGCCCAGTTCGCTCTTCTCTCCCTCCCAAGCGCGGG GTTGGGTGCCTGATCCCGGACCACACAAGCGCTCAGGACTCGCCTGACCTCGGAACTGCCTTCGAGAAAG CGTTAGCCTAGGAGATCCGAGCCTCTTTCTCCATACCATAGTTGGTTCAGGTGGTTTCCTCTTCAAACCT TGCGTCTGGCGCGGATAATCCGCGCGGCCGGGCGTTAAGCTCCAGGTCCCTGTCGCTCCGTCGAGGTGGC AAGCCATGGCCGGCTGCTGCTGTTTGTCTGCGGAGGAGAAAGAGTCTCAGCGCATCAGCGCGGAGATCGA GCGGCAGCTTCGCCGCGACAAGAAGGACGCGCGCCGGGAGCTCAAGCTGCTGTTGCTGGGAACCGGTGAG AGTGGGAAAAGCACCTTTATCAAGCAGATGAGGATAATCCATGGGTCTGGCTACAGTGATGAAGATAGAA AGGGCTTCACGAAGCTGGTTTACCAAAACATATTCACGGCCATGCAAGCCATGATCAGAGCAATGGATAC CCTGAGGATACAATACATGTGTGAGCAGAATAAGGAAAATGCCCAGATCATCAGGGAAGTGGAAGTAGAC AAGGTCACTGCACTCTCTAGAGACCAGGTGGCAGCCATCAAGCAGCTGTGGCTGGATCCCGGAATCCAGG AGTGTTACGACAGGAGGAGGGAGTACCAGCTGTCAGACTCTGCCAAATATTACCTGACGGACATTGAGCG TATCGCCATGCCCTCTTTCGTGCCAACACAACAGGATGTGCTTCGTGTTCGAGTGCCCACCACTGGCATC ATAGAATATCCATTCGACCTGGAAAATATCATCTTCCGAATGGTGGATGTTGGTGGCCAGCGATCTGAAC GACGGAAATGGATTCACTGCTTTGAGAGTGTCACCTCCATCATTTTCTTGGTTGCTCTGAGTGAATATGA CCAGGTTCTGGCTGAGTGTGACAATGAGAACCGCATGGAGGAGAGCAAAGCCCTGTTTAGAACCATCATC ACCTACCCCTGGTTTCTGAACTCCTCCGTGATTCTGTTCTTAAACAAGAAGGATCTTCTAGAGGAGAAAA TCATGTACTCTCATCTAATTAGCTACTTCCCAGAGTACACAGGACCAAAGCAAGATGTCAAAGCGGCCAG GGACTTTATCCTGAAGCTGTATCAAGACCAGAATCCTGACAAAGAGAAGGTTATCTATTCTCACTTCACT TGTGCTACAGACACCGAGAATATCCGCTTTGTGTTTGCTGCTGTCAAAGACACAATCCTACAGCTAAACC TACGGGAGTTCAACTTGGTGTAAATGGAGGGCCTACTCCTCCGAGACAGAGGGTGATCTGAGCCCTTCCT GCCTGATCTACAAGTGCTTCTGGACCAGGACCTAAGGACATTATGTAGCCCACAGGGACAGAGATGGGTA GTGCAATGTGAAAAATACTTCACCAACCCTTTTAAGTGTCTTTAATTCTTCACTGTCTAACTCTTTTCTC GCCTTTTGGTTGAACGATTAGGTATCATTTTTGAGTGGTTCCCCCTCTCCTATTTTTAAACTAGTGTTCA ACAGTTATTAAAAATCATGCAAAAAAATTCATGCAACAGAGGGACTTGTTAATTTACAAATCATGCCTTC AGACCTCTGAGATTCATTTAGGTGACTTAAACATCTTCATTGGTTTGATAATTTTAAAATAAATCTTTTA AGAAACTTTTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5375817 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5375817 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19281 same embryo
	EMAGE:19284 same embryo
	EMAGE:19285 same embryo
	EMAGE:19283 same embryo
	EMAGE:19280 same embryo
	EurExpress:euxassay_002103 same experiment
	MGI:4825126 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source