EMAGE mouse gene expression spatial database

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EMAGE:19289

Phf21a PHD finger protein 21A ( MGI:2384756)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002083. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002083_01	euxassay_002083_02	euxassay_002083_03	euxassay_002083_04

euxassay_002083_05	euxassay_002083_06	euxassay_002083_07	euxassay_002083_08	euxassay_002083_09

euxassay_002083_10	euxassay_002083_11	euxassay_002083_12	euxassay_002083_13	euxassay_002083_14

euxassay_002083_15	euxassay_002083_16	euxassay_002083_17	euxassay_002083_18	euxassay_002083_19

euxassay_002083_20	euxassay_002083_21	euxassay_002083_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19289_wholemount_strong.wlz

19289_wholemount_moderate.wlz

19289_wholemount_weak.wlz

19289_wholemount_possible.wlz

19289_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19289_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4643
Entity Detected:	Phf21a, PHD finger protein 21A ( MGI:2384756)
Sequence:	sense strand is shown >T4643 CCACGCGTCCGGCCAGCAGCAGGAGGGGGACTGGAGAGCTGAAGGAGCGCCAGCTTCTCCAGAATTGGAC TTCTCAGAACCTTTAATATGCTAATGTGCATTGTGAATCTCCAAGATGGGGATATGATATGCAGCATTCT TGAATACTTCTAATGACAGGGAGCCCACTACCTCATAAGCTGCAGCAACAAGAGGAGCTTGTAATTTTAA ACGGAGGCTGAAGACACTGTAGAATTAGCAGACATAAGAGAGCGTGGAGAAGGCAGAGGATGGAGTTGCA GACTCTACAGGAGGCTCTTAAAGTGGAAATTCAGGTTCACCAGAAACTGGTTGCCCAAATGAAGCAGGAT CCACAGAATGCTGACTTAAAGAAACAGCTTCATGAACTCCAAGCCAAAATCACAGCTTTGAGTGAGAAAC AGAAAAGAGTAGTTGAGCAGCTGCGCAAGAACCTGATAGTCAAACAAGAGCAGCCGGACAAGTTCCAGAT ACAGCCATTGTCACAGTCTGAAAACAAACTACAAACAGCACAGCAGCAGCCACTACAGCCACTGCAGCAG CAGCAACCGCAGCAACCGCAGCAGCAGCAGCAGCAGCAGCAGCAGCACGCCCAGCAGTCAGCAGCAGCAC CGCCTAGTCTAACTGCATCACAGGTTCAAGCAACACCTCCTCAGCCCATCAAAGTACCACAGTTTATCCC TCCTCCTAGACTCACTCCACGCCCAAACTTCCTTCCACAGGTTCGACCCAAGCCTGTGGCCCAGAATAAC ATTCCTATCGCTCCAGCGCCTCCTCCCATGCTTGCAGCTCCTCAGCTTATCCAGAGGCCTGTCATGCTGA CCAAGTTTACACCCACAACCCTCCCCACATCCCAGAATTCCATCCACCCTGTCCGTGTTGTCAATGGACA GACTGCAACCATAGCCAAAACGTTCCCCATGGCCCAGCTCACCAGCATTGTCATAGCTACTCCAGGGACC AGACTCGCTGGACCTCAGACTGTACAGCTTAGCAAACCAAGCCTGGAGAAACAGACAGTTAAATCCCACC CAGAAGCAGAGGAAAAACAAGCAGAGAGCCGTACCGTTACCCCACCTGCTGCACCAAAACCAAAACGAGA AGAGAACCCTCAGAAACTTGCCTTCATGGTGTCTCTAGGGTTGGTAACACATGACCATCTAGAAGAAATC CAAAGCAAGAGGCAGGAAAGAAAGAGAAGAACAACAGCAAACCCGGTGTACAGTGGGGCTGTCTTTGAGC CAGAGCGTAAGAAGAGTGCAGTCACTTACCTTAACAGCACAATGCATCCTGGGACGCGGAAGAGAGCCAA TGAGGAACACTGGCCAAAGGGCGATATCCATGAGGATTTTTGCAGTGTTTGCAGAAAAAGTGGCCAGTTG CTGATGTGTGACACGTGTTCCCGTGTGTATCATCTGGACTGCTTAGAGCCGCCCCTGAAAACAATTCCCA AGGGCATGTGGATCTGTCCCAGATGTCAGGACCAGATGCTAAAGAAGGAAGAAGCAATTCCTTGGCCTGG AACCTTAGCCATTGTTCATTCCTACATCGCCTACAAAGCAGCAAAAGAAGAGGAGAAACAGAAGCTACTT AAGTGGAGTTCAGATTTAAAACAGGAGCGGGAACAACTGGAGCAGAAGGTCAAAGAGCTCAGCAGTTCTA TAAGTAAATGTATGGAGATGAAGAGCAGCATCCTGGCGCGGCAGAAGGAGATGCGCAGCTCCCTGGACAA GGTGAAGCGGCTCATCCGCCTTGTGCATGGCGTCGACCTCTGCAGACCTGTGGACTCAGAGGCCACGGCG GGGGCCCTCTCCAATGGCCCAGACTGCACTCCCCCTGCCAACGCCGCCTCCACACCCGCCCCTTCCCCCT CCTCGCAGAGCTGCACAGCGAACTGTAACCAGGGGGAAGAGACTAAATAACAGAGCCCTCGAGGCGGACA TGCCACAGTCCTGGTGGCGAGGAGACGCTAGAAGAGCAAAGCCGGATTTCTTCTGGAAAGTACAGAGTTC TTTTGGTTCTTTGGTTCCAGAGAGAGAGAAGATGCTTGTGCCAGGTGGCACCCGAGTTTGCCAATTGATT CTTGTTATTCTGTGTGTACATGCAAAGACTGGACCATGTTACATGAAATAGTGCCAGCTGGAGGTTCTTT GCCAGCACCATGCCAAGTGACATAATATATTACTCTCTCTATTATACACCAGTGTGTGCCTGCAGCAGCC TCCACAGCCACGGTGGGTTCGTTTTTGTTTTGTTGGGTGGGGAGCAGGGACGGGCGGAGGGAGGAGAGCA GGTTTCAGATCCTTACTGGGAGCCGTTTGTTTAGGTAGAAAGACAAGTCCAAAGAGTGTGTGGGCTTTCC TGTTCCTAAACTTTTCACTACCATAAAACCAAAAAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5008954 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5008954 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19286 same embryo
	EMAGE:19287 same embryo
	EMAGE:19288 same embryo
	EurExpress:euxassay_002083 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source