EMAGE mouse gene expression spatial database

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EMAGE:19344

Tfap4 transcription factor AP4 ( MGI:103239)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002143. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002143_01	euxassay_002143_02	euxassay_002143_03	euxassay_002143_04

euxassay_002143_05	euxassay_002143_06	euxassay_002143_07	euxassay_002143_08	euxassay_002143_09

euxassay_002143_10	euxassay_002143_11	euxassay_002143_12	euxassay_002143_13	euxassay_002143_14

euxassay_002143_15	euxassay_002143_16	euxassay_002143_17	euxassay_002143_18	euxassay_002143_19

euxassay_002143_20	euxassay_002143_21	euxassay_002143_22	euxassay_002143_23	euxassay_002143_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19344_wholemount_strong.wlz

19344_wholemount_moderate.wlz

19344_wholemount_weak.wlz

19344_wholemount_possible.wlz

19344_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19344_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5525
Entity Detected:	Tfap4, transcription factor AP4 ( MGI:103239)
Sequence:	sense strand is shown >T5525 CCGGCCGATTCTGCACCTCCGTCCGGCGCTGCCCTTTGATTCGGATTTCCATCTTGCATTCTCCGGCTGA CCGCGAGACCTGGCTCGTGCACAGGAGGGGGGCCTATCGCTATGGAGTATTTCATGGTGCCCACTCAGAA GGTGCCCTCTTTGCAACATTTCAGGAAAACAGAGAAAGAAGTGATAGGAGGGCTCTGTAGCCTAGCCAAC ATTCCACTGACCCCCGAGACCCAGAGGGACCAGGAAAGGCGGATTCGACGCGAGATTGCCAACAGCAACG AGCGGAGGCGCATGCAGAGTATCAACGCGGGCTTCCAGTCCCTCAAGACCCTCATTCCCCACACAGATGG AGAGAAGCTCAGCAAGGCAGCCATTCTCCAGCAGACAGCAGAATACATCTTCTCTCTTGAACAGGAGAAG ACTAGACTCCTGCAGCAGAACACACAGCTCAAGCGCTTTATCCAGGAGCTGAGTGGCTCATCCCCGAAGC GGCGGCGGGCGGAAGACAAGGATGAGGGCATTGGCTCACCCGACATCTGGGAGGATGAGAAGGCTGAGGA CCTTAGGAGAGAGATGATCGAGCTGCGACAACAGCTGGACAAGGAGCGCTCCGTGCGGATGATGCTGGAA GAGCAGGTGCGCTCCCTCGAGGCCCACATGTACCCGGAAAAACTCAAGGTGATTGCCCAGCAGGTGCAGC TGCAGCAGCAGCAGGAGCAGGTGCGGCTGCTGCACCAGGAGAAGCTAGAGCGGGAACAGCAGCACCTACG GACCCAGCTCCTGCCTCCTCCAGCCCCCACCCACCACCCCACAGTGATCGTGCCTGCACCAGCCCCGCCT TCCTCCCACCACATCAATGTTGTCACCATGGGTCCCTCCTCAGTCATCAACTCTGTCTCTACATCCCGGC AAAATCTGGACACCATTGTGCAGGCCATCCAGCACATTGAGGGCACCCAGGACAAACAGGAGCTGGAAGA GGAACAGAGGCGAGCAGTTATCGTGAAGTCTGTCCGCAGCTGCCCAGACGCCCACACCTCTGATACCGCC TCGGACTCCGAGGCCTCGGACAGCGACGCCATGGACCAGAGCCGGGAGGAGCCTTTGGGGGACGGGGAGC TTCCCTGACCACCCCCAGCCCTCCTCTCCCTCCTGGGGGCTAGAGGGGGCCGGGGTAACAACAGGGAGAA ACATGGGTGAATGAGAAATTTTTACAAAATTATGATGTCATTTGGGTCTCTTTTATGACCTCCTTTTCAA TACTGTAAGTCGACCTTTGATCGAAGCCACCGGAGCCCAGGTCCCGGGGTTGTGGTGGTATATAGCACGC GTGGGAGCATCGGGACTCCAGGGCTGGACCTCGGCCCTGGGGCCAGGGAAGCTGACACTGAGGTGCCTAG CCTCTCTCGCCAAAAAGCTTTTTTATTTTTGTTTTGTTTTTGTTTTTTCCTTCCATTTAAACGTGTTTTT AAGAACAGGGAAAATCAAACAAAACCCCAAGGTATTTGCTCCCGCCCCAGAGCCAGCCCGAGGCTGTCGT TCCTCACCCCTTCCCTCTGTCGGGTTATCTTTTTCTCTTTTAAGCACTTACATGGGTTGGGGTCAGGCTG GGTTGGGGCGTTCTGGGGACCCGGGGGATGGGGGATGGGGATCGGTGATCGACATTGCTTCTCAGTTGGG GTTTGCTGCTGCAGTTGTCCCCTCCCTCTCCCCACCTCAGCACCAGAATTGGCCACTCTCTGCCTTCCCC TCCAGGGTTCCTGTTATTGACCCCAAAAATGTCTTTGTCTCTCTTTTTGTTTTGTTTGTTGTTGGTTTTA TTTTGGTTTTGGTTTCTGTATTTTTTTAATTTTAATTTTTCTTTTCTTTTTTTTCTTTTCTTTTTTACAA TTTTGAGGTCTTTGTGTCCAAGGAGAAGCTATTATATTTTGTTAAGAAAATGGGGGTAGGGAACAAAATA CCAAGAGGCCATTGTGCCTTTGTAAAGAAACAAAATAAAGTTTGTACTTGGTTTTTTAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:6438955 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6438955 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19347 same embryo
	EMAGE:19346 same embryo
	EMAGE:19345 same embryo
	EMAGE:19343 same embryo
	EMAGE:19342 same embryo
	EurExpress:euxassay_002143 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source