EMAGE mouse gene expression spatial database

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EMAGE:19354

Fam63b family with sequence similarity 63, member B ( MGI:2443086)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002097. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002097_01	euxassay_002097_02	euxassay_002097_03	euxassay_002097_04

euxassay_002097_05	euxassay_002097_06	euxassay_002097_07	euxassay_002097_08	euxassay_002097_09

euxassay_002097_10	euxassay_002097_11	euxassay_002097_12	euxassay_002097_13	euxassay_002097_14

euxassay_002097_15	euxassay_002097_16	euxassay_002097_17	euxassay_002097_18	euxassay_002097_19

euxassay_002097_20	euxassay_002097_21	euxassay_002097_22	euxassay_002097_23	euxassay_002097_24

euxassay_002097_25	euxassay_002097_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19354_wholemount_strong.wlz

19354_wholemount_moderate.wlz

19354_wholemount_weak.wlz

19354_wholemount_possible.wlz

19354_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19354_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 13 14 15 16 17 18

brain

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

facial vii ganglion

strong

regional

strong expression: see section 05 06 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 09 19

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 10 18 19 20 21 22 23

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 07 moderate expression: see section 20

spinal cord

moderate

homogeneous

moderate expression: see section 12 13 14 15 16 17 18 19

dorsal root ganglion

strong

regional

strong expression: see section 11 12 13 17 18 19 20

naris

moderate

regional

moderate expression: see section 12 13 14 16 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 13 15 16 17 18

esophagus

moderate

regional

moderate expression: see section 16

rectum

moderate

regional

moderate expression: see section 15

liver lobe

strong

regional

strong expression: see section 02 03 04 05 06 moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26

urethra of male

moderate

regional

moderate expression: see section 15 weak expression: see section 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5503
Entity Detected:	Fam63b, family with sequence similarity 63, member B ( MGI:2443086)
Sequence:	sense strand is shown >T5503 CGCTAGGGCGCTGGAGCTGCTACAGAGCGGCTGTCATGGCGTCGGAGGCGCTTGCTGAGGAGAGCCGGCC GCGGTGTCTATAGGACCAGGGGCGGGCGGCCGGGGATGGAGAACAGCCCTGACAGCCCACAGCCTCTGGA ACTAGGGGTGGCGGCCGGGCGAGTGTCGCCGCCGGAAGGGCGACGGCGCGGCGGGCGCGAGGCTGAGGAT GGTCCCGCTGGACGCGCGGTGGACAGCGGCGGGCAGGGGGCGGCGGCGGCGGCAGCGCGGAGCAGCCTCG GGGACCCGACGTCCCCCTCGCAGCTCGGATGCGGCGCGGGCTCGGACTTGAAGGACGGCGCGTCGTCGAG CCCTGCCGCCTCGGAGGTGCCTTCAAGAGGGCAGCACAAGGTGACCGCCTCCCCGGAGCTGGCCGAGGCC GCGGCGGGCCGAGGGTCGGGGCCAGTGGGCGACACGGGCACCTGCAGGGTGGAGCAGGCGGCGGAAGAGC CCAGCAGCACCGGCGCCCCGAGCAGCAGCTGCAGCGAGCCGAGCCCCCCTGGGGACTCGCCCAGCTTGGA CTCCCTGGAGTCTTTCTCCAACCTGCATTCTTTCCCCAGTAGCTCTGAGTTCAATAGTGAGGAGGGAGCA GAGACCAGGGTCCCCGAGGACGTGGAGGAGGGCGCGGCGGGGCCGCCCAGGGCTGCACCTCTATGCAAAG AGGAGGAGGAGGACCCGGCTCAGGTGCTAGCGGCCTCCAAAGAACGCTTTCCCGGACAGTCGGTGTATCA CATCAAATGGATCCAGTGGAAAGAGGAGAACACGCCCATCATCACCCAGAATGAGAATGGACCCTGCCCT TTGCTGGCCATCCTCAATGTTTTGCTTCTCGCCTGGAAGGTGAAATTTCCACCAATGATGGAAATCATAA CTGCGGAGCAGCTGATGGAATATTTAGGTGATTATATGCTTGAGGCAAAGCCAAAAGAAATATCAGAAAT TCAACGTGTAAATTATGAACAGAATATGAGTGATGCTATGGCAATCTTGCACAAACTGCAGACAGGCCTG GACGTGAATGTCAGGTTCACTGGGGTACGAGTTTTCGAGTACACGCCAGAGTGCATAGTCTTTGATCTTC TAGATATTCCTTTGTATCATGGGTGGTTAGTGGACCCTCAGATTGATGACATTGTAAAAGCTGTTGGCAA CTGCAGCTACAACCAGCTGGTAGAGAAGATCATCTCCTGTAAGCAGTCAGACAACAGCCAGTTGGTTAGT GAAGGCTTTGTAGCTGAGCAGTTTCTAAACAATACAGCCACTCAACTGACGTACCATGGATTATGTGAAC TGACTTCAACGGTTCAGGAAGGAGAGCTCTGTGTGTTCTTTCGGAATAATCATTTTAGCACCATGACAAA ATACAAGGGTCAACTGTATTTGTTGGTAACAGACCAAGGGTTTCTTACTGAAGAAAAAATTGTCTGGGAA AGCCTCCACAATGTAGATGGTGATGGAAATTTCTGTGACTCAGAATTCCACCTTCGGCCTCCTTCAGATC CTGAGACTGTATACAAAGGTCAACAAGACCAAATAGATCAGGACTACCTTATGGCACTGTCACTGCAACA AGAACAGCAGAGCCAAGAAATCAATTGGGAGCAAATTCCAGAAGGAATCAGTGACTTGGAGCTGGCAAAG AAACTCCAGGAAGAGGAGGACCGACGGGCCTCTCAGTACTACCAGGAGCAGGAGCAGGCTCAGGCTGTTG TGACTACCACCACCCCTTCCACACAGGCCCAGGGCCAGCCAGCACAAGCCTCTCCATCAAGTATAAAACA GCCGGGAATAGTGAACGGAAACGCAAGGAACCTCGGGAAAAAGACAAAGAGAAAGAAAAGGAAAAAAATA GCTGTGTGATTTTGTAGGGAATGCTGACGTCTGTGGGAACCACCTATGCAGATGACGGAAAGGAAGACCC AGTCCCTACCGTCTGTGCCTGATCTCCCAATGGATTTTGCTCATGTTTTCCAGGGGAAGGTTGTTACTTT GTTACAATCAGCTTTTTCAAGTTACACCATACACTCTCTATGAGCTGGAGTTTTATGTTACAGGTCGGAA CTGGTGTGTGTTGTCACTGTAGCCAAATGAGAATATCACTCCTATTTTGTGTCTAGTGACATTGCAACTC CAATCAGTTAGTAAGCTATTGTGTTCTGTGTGACAATGGATGGAGGTCACTGAATAAGTTTGATCCTTCT CAAGATGTAATTGTTAGCACAACTATTTCTGAAACTTTACATTGTTAAATTATATCCTAATCCATGAGGA ACGAGGACTTTAATATAGGGGAGGGGGAGAGAGCAGGAATGCTGACCCTTCATTGTATATTGAACTGGTT CAGCCCTTGGACAGCTTTACCTTTCTTTAGTAATGTACATTTTAGCTACTACCTTGAGAACTGATAATGG AGCTTGGATCCAATTTAGATAGAAAAAATATTTTTTATTTTCAAATAGCAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:6395358 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6395358 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4824751 same experiment
	EurExpress:euxassay_002097 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source