EMAGE mouse gene expression spatial database

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EMAGE:19357

Tubgcp3 tubulin, gamma complex associated protein 3 ( MGI:2183752)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002145. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002145_01	euxassay_002145_02	euxassay_002145_03	euxassay_002145_04

euxassay_002145_05	euxassay_002145_06	euxassay_002145_07	euxassay_002145_08	euxassay_002145_09

euxassay_002145_10	euxassay_002145_11	euxassay_002145_12	euxassay_002145_13	euxassay_002145_14

euxassay_002145_15	euxassay_002145_16	euxassay_002145_17	euxassay_002145_18	euxassay_002145_19

euxassay_002145_20	euxassay_002145_21	euxassay_002145_22	euxassay_002145_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19357_wholemount_strong.wlz

19357_wholemount_moderate.wlz

19357_wholemount_weak.wlz

19357_wholemount_possible.wlz

19357_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19357_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 10 11 13 moderate expression: see section 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5538
Entity Detected:	Tubgcp3, tubulin, gamma complex associated protein 3 ( MGI:2183752)
Sequence:	sense strand is shown >T5538 GGCGGCGCCGTAGTTAGGGCGCGGGATCCTGGGGCGCGGGGAGGCTGCAGTGCGCAGTCCGGGCGCGGTA ATGGCGACTCCGGATCAGAAGTCGCCCAACGTTCTGCTGCAGAACCTGTGCTGCCGGATCCTGGGCCGGA GCGAAGCTGATGTGGCCCAGCAGTTCCAGTTTGCTGTGAGAGTTATCGGCAGCAACTTCGCTCCAACTGT TGAACGAGATGAATTTCTAGTAGCTGAAAAAATCAAGAAAGAACTTATTCGACAAAGGAGAGAAGCGGAC GCCGCACTGTTTTCAGAGCTCCACAGGAAGCTTCATTCGCAGGGAGTTTTGAAGAATAAATGGTCAATCC TCTACCTCCTGCTGAACCTTAGTGAGGACCCTCGCAAGCAGGCCAGCAAGGTGACCAGCTACGCTTCCTT ATTTGCACAAGCGTTACCAAGAGACGCTCACTCGACGCCTTACTACTATGCCAGGCCTCAGACCCTCCCG CTCAACTACCAGGACCGCAGCACACAGGCACAGAGCTCTGGCAGCCTGGGCAGCAGCGGCATCAGCAGCA TCGGCATGTGTGGCCTTAGTGGCCCCACACCTGTGCAGCCTTTCCTTCCCGGACAGTCTCATCAAGCTCC AGGAGTGGGTGACGGCCTGCGACAGCAGTTGGGGCCTCGTCTGGCGTGGACTCTGACTGGAAATCAGCCT TCTTCACAAACCCCTACCTCCAAGGGTTTCCCAAATGCTCTTTCTCGAAACCTGACAAGGTCAAGGAGAG AAGGGGATCCAGGTGGCACATTGGAAGTTACTGAAGCTGCTCTTGTGAGAGATATTCTCTACGTATTCCA GGGCATTGATGGCAAAAACATCAAAATGAGCAGCACTGAAAACTGTTACAAAGTGGAAGCGAAGGCAAAC TTAAACAAATCTTTGAGGGACACAGCAGTCCGGCTTGCTGAGTTGGGATGGTTGCATAATAAAATCAGAA AGTATGCTGACCAGAGGAGTCTGGACCGCTCATTTGGACTTGTAGGTCAGAGTTTTTGTGCCGCATTGCA CCAAGAGCTCAAAGAATACTACAGGCTCCTCTCTGTTCTGCACTCTCAGCTCCAGCTAGAAGATGATCAG GGTGTGAATTTGGGACTAGAGAGTAGCCTCACACTGCGGCGCCTCCTGGTTTGGACATATGACCCCAAAA TAAGATTAAAGACCCTTGCTGCGCTGGTAGATCACTGCCAAGGACGAAAAGGGGGTGAGCTGGCCTCTGC TGTTCATGCCTATACAAAGACAGGGGACCCCTACATGAAGTCCCTGGTGCAGCACATTCTGAGCTTGGTG TCTCACCCTGTCCTGAGCTTCCTCTACCGGTGGATATATGATGGGGAGTTGGAGGACACTTACCACGAAT TTTTTGTGGCGTCTGATCCAACAGTTAAAACTGACCGTCTATGGCACGACAAGTATACCCTGAGGAAGTC AATGATCCCCTCCTTCATTACGATGGACCAGTCTAGGAAGGTCCTTTTGATAGGAAAGTCAATAAATTTC TTGCACCAAGTTTGTCATGATCAGACACCTACTACAAAAATGATAGCAGTGACGAAGTCGGCAGAGTCAC CCCGTGACGCTGCGGATTTATTCACAGACTTAGAAAACGCATTTCAAGGGAAAATTGATGCTGCTTATTT TGAGACCAGCAAATATCTGTTGGATGTCCTCAATAAAAAGTACAGTCTGCTGGAACACATGCAGGCCATG CGACGGTACCTGCTGCTTGGCCAAGGAGACTTCATACGGCACTTAATGGATTTGCTCAAACCAGAACTTG TTCGGCCAGCAACAACTTTGTATCAGCATAACTTGACGGGGATTCTTGAAACTGCTGTGAGAGCCACCAA TGCTCAGTTTGACAGCCCGGAGATCCTTAAGAGGCTCGATGTGAGGCTGCTGGAGGTCTCTCCTGGTGAC ACTGGGTGGGATGTCTTCAGCCTGGATTACCACGTCGATGGACCAATCGCCACTGTGTTCACACGAGAGT GCATGAGCCACTACCTGAGAGTGTTTAACTTCCTGTGGAGGGCCAAGCGCATGGAGTACATCCTCACTGA CATCCGGAAAGGGCACATGTGCAACGCGAAGCTGCTGAGGAACATGCCGGAGTTCTCTGGGGTGCTTCAC CAGTGCCACATCCTGGCCTCAGAGATGGTGCACTTCATCCACCAGATGCAGTACTACATCACCTTTGAGG TTCTCGAGTGCTCCTGGGATGAGCTGTGGAACAGAGTGCAGCAGGCCCAGGACCTGGACCACATTATTGC AGCACACGAGGCGTTCCTGAACACCATCACCTCCCGCTGTCTCCTGGATAGCAACTCCAGGGTGCTTTTA AACCAACTGAGAGCCGTGTTTGACCAAATCATTGAACTTCAGAATGCCCAGGACGTGATGTACAGAGCAG CTCTGGAGGAGCTGCAGAGGCGTCTGCAGTTTGAAGAGAAAAAGAAACAGCGTGAAATTGAGGGCCAGTG GGGAGTGACTGCGGCAGAGGAAGAGGAGGAGAACAAGAGGATCCGAGAATTCCAGGACTCCATACCAAAA ATGTGCTCACAGTTGCGAATATTGACGCACTTCTACCAGGGTGTGGTGCAGCAGTTTCTGGTGTTACTCA CGACCAGCTCTGATGAAAGTCTCCAGTTTCTCAGCTTCAGATTGGACTTCAACGAACATTACAAAGCCAG AGAACCCAGGCTCCGGGTCTCTCTGGGCAGTAGGGGGCGGCGCAGTTCCCACACGTGAGGCTGGCTTGGG TTAGTACATGCTTACTCAAGCCTGGCCCCTCAAGCAGCTGTGAGCTGCAGGTGCCCCCTGTCTACACACA GGCCCCAGTGTTCAGCACTGTGTCTGCTGGAAGAAGTCTTGTTATCCACTGGACTTGCGTGTCTGTAACG CTGCTGAGTTCAACAGGCCACTTACCTACCATGTAGTCTCCGTAGTCGAAAGACACTGTGATGTGCTGGG ACATCTTATTTCCACCGTGTAATTTATATTTCAACAACAACAAACTGTTTCTTGCCACAAAAAAACTAGA CCATAAATTAAGAGTAATGTCACTGCTGTTTTTTAGCATTCTCTGTGAGACAGTTTTTGTTATCATGATG ACACTAAAATTGAAACATGTCTAAGAAAGTCCACAGGCTGTTTTACTCTGGCTTCAGTGTTTGTAACCCT CACCTGCGTGAGGTCATCACTACCCACGCGTCATCTCCACACACTGTCCTCTCTCCCTCCCGAAGTTTAC ATTTTTATATTTGCTTTACTGTTTCAGATAACATGTCAAACATTTCAAAGAGTGGGAGCCGTGGAGGGAG GTGTCCTCAGCCTGCCTGGCCGCAGGCCGCTGGCTGAACCAGGCAAGGCTGAGACTGTCTTATTTTCAAA GTTCTGATAAGTGTTAGGAAGAAATCAGCTTGGATTGTGAAGGTTTCCCTTCTCCTTGCCTATTTTTTCA CTGTAAATATTTATATACTACTGACCAGATGACGGAGGGGACTGGGTTTTGGTTTTTTTTGTAAAAATGT CTTATCCAGTCACATAATTATGCCTTTTTTATGTTGCATAAGTGAAAACTTAGAGAATTAAAAACCATTA TCTTTCAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6466057 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6466057 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19358 same embryo
	EMAGE:19355 same embryo
	EMAGE:19356 same embryo
	EurExpress:euxassay_002145 same experiment
	MGI:4829019 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source