EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:19358

Golph3l golgi phosphoprotein 3-like ( MGI:1917129)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002139. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002139_01	euxassay_002139_02	euxassay_002139_03	euxassay_002139_04

euxassay_002139_05	euxassay_002139_06	euxassay_002139_07	euxassay_002139_08	euxassay_002139_09

euxassay_002139_10	euxassay_002139_11	euxassay_002139_12	euxassay_002139_13	euxassay_002139_14

euxassay_002139_15	euxassay_002139_16	euxassay_002139_17	euxassay_002139_18	euxassay_002139_19

euxassay_002139_20	euxassay_002139_21	euxassay_002139_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

19358_wholemount_strong.wlz

19358_wholemount_moderate.wlz

19358_wholemount_weak.wlz

19358_wholemount_possible.wlz

19358_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:19358_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5539
Entity Detected:	Golph3l, golgi phosphoprotein 3-like ( MGI:1917129)
Sequence:	sense strand is shown >T5539 GGAAGCTGATGTGTTATTCCCTCTCTGCAACGAAGGATCAGGAAGTTTGTACTCTCTGTGTGGCTGAGAA GTTTTTCACCTACTGGAACCCGCCTAGGGTACGGGAAACAGGTGCCCTCCAAAACGAGAGCGCGAACTGC AGCCTACGTCCCACTGCAGCTCAGGAGCAACGTCAGGTAAGAATGACAACATTAACTCATAGAACCCGTC GAACTGAAGTAAGTAAGAGCTCTGAAAAGAAAATAGAAAGTGAGGAAGACACTAATCAAGAAAGGAGTCC AGATAATGAAGACCCTGGAGACTCTAAGGATATCCGCCTCACTCTTATGGAAGAAGTATTGCTCCTGGGG CTAAAAGATAAAGAGGGCTACACATCTTTCTGGAATGACTGTATATCATCTGGACTGCGAGGAGGCATTC TGATAGAGCTGGCCATGCGGGGTCGAATCTATCTGGAACCTCCCACCATGCGTAAGAAGCGACTCCTAGA CAGAAAGGTCCTGCTGAAGTCAGACAGCCCAACAGGTGATGTTTTGCTGGATGAGACGCTCAAACACATT AAAGCAACTGAGCCAACCGAAACTGTGCAGACGTGGATCGAGCTACTCACTGGTGAGACATGGAACCCTT TCAAATTACAGTACCAGCTGAGAAATGTACGAGAACGAATTGCCAAGAACCTGGTGGAAAAGGGCATTCT AACCACAGAGAAGCAGAATTTCCTGCTCTTCGACATGACCACTCATCCAGTGACCAATACGACAGAGAAA CAGCGACTAATGAAAAAGCTTCAAGACAGTGTGCTAGAACGGTGGGTAAATGACCCTCAGCGCATGGACA GGCGAACACTGGCCCTCCTGGTGCTGGCCCACTCTTCTGATGTGCTGGAGAATGTCTTCTCTTGTCTGAC AGATGATAAGTATGATGTGGCAATGAATCGAACCAAGGACCTAGTAGAACTGGACCCTGAAGTGGAGGGG ACAAAGCACAATGCTACAGAGATGATCTGGGCTGTGCTGGCAGCCTTCAACAAATCCTAAAGCCAGCAGG CGGGTGTCTCCTTTTCCCGGCTGGCTGGTGACTCTCAGAGACCCAATCACTGAGTTCAGTGTGATAAGAT GCTATCATCATTTCCCCACTTTTTGAATCAGACTCCTGACTTGAGGAGGAAAACTTCATGGCCTCTCTGT CCATCGGCCATGACCTTCTAAGCAGACTGTTTCTCCTCACTTCCACTCTGTCTGAATGAAAGGGGTGAAC CCACACACATCTAATAAACAACTCCCATTTCTGGGTTTAGTTTTAACCAGTGTGGTTCTGGATAAACAAG AGTTCCTGTGTGGGGATTAGAGTTTTCCAGCAACAAAATGTGAAGGAAATGAACAGTAAACCATATTCAA TTTTTTTTCTCCCTTATTCATGTTTTCTCAGGCTTTGTTTCTCCGCTCTGTTAAGCAGTGACACTTCCTG AAATCACTGACTCTTTGAATGAGAGGCAGTGGGAACAAGACTAATTTTTGAAGCAAATGGTTCCTGAGAT TATACCTCCCACCTGTGATTTTCTCATCTTCACATAATGAAAACACTCCAAGTTTGATGACCCGAATCCC TTAGTTCTCAGAGCAGAAATGTATTCTACACTCCTTTTTAGCTTTGTCTGGCTCCCACACTCATTAAGAT TCTGAATTGAAAAAGCAGTGTGCCTCAGTCACCTCCTCCTTACAGCCAGCATTAGGACCTGTAGAGAAGT GGGGCAACATGAGACTTCACATCCATGAAACCAAGTGTCATCTGAGCCCTGAAGAGAGAGTGGCAGCTAG ATTCAGGGCTAGCCTTGGCTATATGAGACCATCTCGCATTGAAAAATTAAAGACTTTTCACATATCCTTC AGTATGTGACTGTTCTGTTTGCTCTAGACTACTTTAAATATCTCTACTCTGATAAAATTAAGGGAATTAA GTTTCAAGTCCCAAATTACAGCATTGAGTCCCAACAATGTAGCCAACCTCCATATTTGCATAGTCCCTGT TTTTATTTCCTACATAAATGGATAAATTTTCCCTGTATTTAAGGGCTTTGGCAGTAATTCTGTCTATATT TAATCTATTGTCACCCTCTAAATTGTGTCTTCTTTCAACACAGCCATCCTGGCCAAATCCCTTAAAATGT TTTTGCTTCAGTAGAGAGGATGAAGAGGGTGGAGAGATCCTATTTAGATGGCCAGTTGAGTGTCACCAGC AGCAGATGCAAAGCCTAAGCTCAAGGGTTGTGAAGAGGCCAGCACTGTGTTGTGCAGAGTTCAAGCACCC TGTTGACCACTGGGGAGAGACAGGCTTTCACACAGCCACCACAGATGACTTGGACTAATACCAAATCCTT CTAATCAGAAATAAATTATAACCTCATCGTTCCCTTGAACCTTCCTAGGTCCATGAAAGTTCTCCAGCTT ATTGTTATTGTTTTCATCTGACTCTTAGATGAGTAGAGATAAAAGCTGTCATTTGAGAAGCTAAGCAGAG CCTTAGTCTGGCTTTCTAGAAGGGCAAAAGCCAGGCCATGTATAATCTAGCAGCTCATATTATCTTCCCC TTAGAACAATTCATAGTGAATTGAAACCAGATTTTCCTTTGAAAGCTTTAGCTTGTTTGGGGAATAGAAG TTGAATTCTTTCTTGCAACTCTCAATGTTTATTTTTATATGTCCATACAATGAGAATGTCTTCACTTCTA ATCTGTGTAATGTTCACTGCCAAACACTCATTTAGTGTATATTGCCTTCACATTTCTGTATAATAAAGAT CAATAATTGTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6465983 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6465983 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:19357 same embryo
	EMAGE:19355 same embryo
	EMAGE:19356 same embryo
	EurExpress:euxassay_002139 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source